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Method for detecting IgG content

A detection method and content technology, which is applied in the detection field of IgG content in bovine colostrum, can solve the problems of low accuracy and error, and achieve the effect of accurate detection results

Inactive Publication Date: 2012-06-20
JIANGSU XINGCHI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the main methods for detecting IgG content in China are: single immunodiffusion method, HPLC method, and electrophoresis method, but the accuracy is not high and there are large errors

Method used

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  • Method for detecting IgG content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A detection method for IgG content, comprising the steps of:

[0025] Preparation of standard curve: Dissolve standard IgG in buffer to prepare IgG standard solution, the concentrations are 1mg / ml, 0.5mg / ml, 0.25mg / ml, 0.1mg / ml, 0.05mg / ml respectively, using IgG-SPA complement As determined by the binding method, the base numbers of red blood cells were 177, 403, 742, 1632, and 2980, respectively. The minimum detection level is 0.005mg.

[0026] Precisely prepare 1 mg / ml IgG sample solution with buffer solution for the sample IgG, accurately draw 100 ul into the sample tube, put it into an ultrasonic cleaner and mix for 15 min. Prepare an SPA solution with 5 times the concentration of the IgG sample solution, mix it fully with the IgG sample solution in the sample tube, and carry out the IgG-SPA composite reaction. In order to speed up the reaction, it can be placed in an ultrasonic cleaner and mixed for 10 minutes to generate an IgG-SPA composite solution. At the sam...

Embodiment 2

[0028] A detection method for IgG content, comprising the steps of:

[0029] Preparation of standard curve: Dissolve standard IgG in buffer to prepare IgG standard solution, the concentrations are 1mg / ml, 0.5mg / ml, 0.25mg / ml, 0.1mg / ml, 0.05mg / ml respectively, using IgG-SPA complement As determined by the binding method, the base numbers of red blood cells were 204, 398, 750, 1666, and 3202, respectively. The lowest detection level is 0.008mg.

[0030] Precisely prepare the sample IgG with buffer solution to make 0.5 mg / ml IgG sample solution, accurately pipette 200ul into the sample tube, put it into the ultrasonic cleaner and mix for 5min. Prepare a SPA solution with 7 times the concentration of the IgG sample solution, fully mix it with the IgG sample solution in the sample tube, and carry out the IgG-SPA composite reaction. In order to speed up the reaction, it can be placed in an ultrasonic cleaner and mixed for 20 minutes to generate an IgG-SPA composite solution. At th...

Embodiment 3

[0032] A detection method for IgG content, comprising the steps of:

[0033] Preparation of standard curve: Dissolve standard IgG in buffer to prepare IgG standard solution, the concentrations are 1mg / ml, 0.5mg / ml, 0.25mg / ml, 0.1mg / ml, 0.05mg / ml respectively, using IgG-SPA complement As determined by the binding method, the base numbers of red blood cells were Ig1, 377, 756, 1647, and 3120, respectively. The lowest detection level is 0.007mg.

[0034] Precisely prepare the IgG sample solution with buffer solution to make a 0.25 mg / ml IgG sample solution, pipette 20ul precisely into the sample tube, put it into an ultrasonic cleaner and mix for 10min. Prepare a SPA solution with 10 times the concentration of the IgG sample solution, mix it fully with the IgG sample solution in the sample tube, and carry out the IgG-SPA composite reaction. In order to speed up the reaction, it can be placed in an ultrasonic cleaner and mixed for 5 minutes to generate an IgG-SPA composite soluti...

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Abstract

The invention discloses a method for detecting IgG content, belonging to the field of medicine detection and analysis. The method comprises the following steps of processing samples, generating an immune complex by samples and SPA and detecting complement binding. By using the method for detecting the IgG content, provided by the invention, the detection result is precise, the defect of great errors caused by different detecting methods in the IgG detecting field is successfully made up, and the IgG content in colostrum can be precisely detected; the method plays a very important role in development and quality of products; meanwhile, the method can be applied to the detection of other immunoglobulins and plays an important promoting role in other biopharmaceutical industries and health care product industries.

Description

technical field [0001] The invention relates to the field of medical analysis and detection, in particular to a detection method for IgG content in bovine colostrum. Background technique [0002] Bovine colostrum is the milk secreted by cows within 72 hours after calving. In the past production practice, it was often discarded as useless. It has been proved in current scientific research that the IgG content in colostrum is very high, which can reach 59 -80mg / ml, has high utilization value. [0003] At present, the main methods for detecting IgG content in China are: single immunodiffusion method, HPLC method, and electrophoresis method, but the accuracy is not high and there are large errors. Contents of the invention [0004] The purpose of the present invention is to provide a detection method of IgG content with high precision and simple operation. [0005] A detection method of IgG content, it comprises the steps: [0006] 1. Dissolve standard IgG in buffer solutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531
Inventor 张慧东卜英俊王彬陈芳顾培华
Owner JIANGSU XINGCHI BIOTECH
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