Guide flow cylinder, cylindrical bioreactor using guide flow cylinder and method for arranging guide flow cylinder

Abstract

The invention relates to a guide flow cylinder, a cylindrical bioreactor using the guide flow cylinder and a method for arranging the guide flow cylinder. The guide flow cylinder is a guide flow cylinder with the cylinder wall having an up and down run-through wave-shaped structure. According to the bioreactor, the guide flow cylinder with the cylinder wall having the wave-shaped structure is arranged in a bioreactor tank, and a top opening and a bottom opening formed by the wavy structure guide flow cylinder are outward-expanded trumpet-shaped openings. The method for arranging the wave-shaped structure guide flow cylinder comprises the following steps: 1, determining the ventilation volume or the ventilation speed of the cylindrical bioreactor according to cultured organism growth demands to obtain the ventilation ratio R:R which is equal to V1 / V2, wherein V1 represents the volume of the gas inlet to the bioreactor per minute, and V2 represents the practical culture solution volume in the cylindrical bioreactor; and 2, determining the wave period number and the amplitude of the wave-shaped structure guide flow cylinder according to the obtained ventilation ratio, and arranging the guide flow cylinder in the bioreactor tank. According to the invention, the liquid mixing frequency can be increased, so the culture solution mixing effect in the bioreactor can be greatly improved, the luminous energy utilization efficiency is improved, and the gas-liquid mass transfer is substantially improved.

Description

technical field [0001] The invention relates to an air-lift cylindrical bioreactor. In particular, it relates to a draft tube and a columnar column using the draft tube to realize the optimal flow rate, turbulent mixing and gas-liquid mass transfer conditions of the organisms cultivated in the reactor by controlling the number of cycles and the amplitude of the concave and convex ups and downs of the draft tube. Type bioreactor and setting method. Background technique [0002] Airlift bioreactors are widely used in plant cell culture and fermentation industry. According to different aeration positions, they are divided into two types: internal circulation type and external circulation type. Driven by gas, the fluidity of the reactor is higher than that of other biological reactions. The reactor is more uniform, and as the liquid carries the air bubbles to circulate in the reactor, good gas-liquid mixing can be achieved. However, at present, the guide tubes in the columnar a...

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Problems solved by technology

[0002] Airlift bioreactors are widely used in plant cell culture and fermentation industry. According to different aeration positions, they are divided into two types: internal circulation type and external circulation type. Driven by gas, the fluidity of the reactor is higher than that of other biological reactions. The reactor is more uniform, and as the liquid carries the air bubbles to circulate in the reactor, good gas-liquid mixing can be achieved. However, at present, the guide tubes in the columnar air-lift bioreactors and photobioreactors are mainly flat straight guide tubes, so that Insufficient mixing of airlift bioreactors when cultivating organisms at high density

In addition, when using this reactor to cultivate photosynthetic organisms, such as microalgae and photosynthetic bacteria, under specific aeration conditions, the mixing ability is limited, coupled with the mutual shielding between organisms, "photoinhibition" will occur when the reactor is close to the light area Phenomenon, while the area far away from the light appears "light limitation" phenomenon, which greatly affects the growth of organisms in the reactor

When increasing the ventilation rate to enhance turbulent mixing, in the current generally used planar straight guide cylinder reactor, the circulating liquid velocity of the culture medium will also increase accordingly, and there will be strong turbulent disturbance at the same time, which will affect the large-scale Some algae and bacteria that are suitable for growth under low flow rate and small disturbance environmental conditions have adverse effects, thereby reducing the production capacity of the reactor

[0003] The current research on the development of biological and photobioreactors is mainly focused on changing the structural parameters of the reactor's built-in guide tube, such as the height-to-diameter ratio of the guide tube, the installation position of the guide tube, and the drop zone and rise formed by the guide tube. Area ratio, etc., to improve gas-liquid mass transfer and mixing in the reactor, but these optimization methods have little effect on the mixing of photosynthetic organisms in the light area, especially the radial mixing in the light direction

At the same time, it does not solve the problem of excessive circulation flow rate of culture medium caused by increasing ventilation.

In addition, the structure of the current planar straight guide tube has little effect on enhancing the mass transfer and mixing in the reactor.

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