Cell culture device with shear force-uniform electric field stimulation

A technology of cell culture and uniform electric field, which is applied in the field of cell engineering and tissue engineering, can solve the problem of not being able to simulate cells in the body, and achieve the effect of preventing bacteria from entering

Inactive Publication Date: 2012-07-04
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the technical problem that the existing methods cannot simulate the physical microenvironment where the cells in the body are in shear force and electric stimulation simultaneously, and propose a shear force-uniform electric field combined st

Method used

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  • Cell culture device with shear force-uniform electric field stimulation
  • Cell culture device with shear force-uniform electric field stimulation
  • Cell culture device with shear force-uniform electric field stimulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Endothelial cell culture stimulated by combined shear force and uniform electric field

[0049] Under sterile conditions, the human umbilical vein endothelial cells were divided into 1 × 10 5 / cm 2 The density is seeded on the lower plate electrode 703, such as indium tin oxide conductive glass. The next day after the cells adhere to the wall, the lower plate electrode 703 seeded with endothelial cells is fixed on the groove of the plexiglass base 705, and then the silicone gasket 704 is placed on the lower plate electrode. On the plate electrode 703, then place the upper plate electrode 702 on the silica gel pad, place the plexiglass cover 701 on the top, and fix the upper plate electrode 702 in the groove of the plexiglass cover 701. In order to facilitate the connection of the plate electrodes with the electrode wires, the upper plate electrode 702 and the lower plate electrode 703 respectively protrude from the cover 701 and the base 705 on both sides o...

Embodiment 2

[0053] Example 2 Osteoblast Culture of Shear Force-Uniform Electric Field Combined Stimulation

[0054] Under sterile conditions, the osteoblasts were divided into 1 × 10 5 / cm 2 The density is seeded on the lower plate electrode 703, such as conductive glass deposited with conductive polypyrrole, the next day after the cells adhere to the wall, the lower plate electrode 703 seeded with osteoblasts is fixed on the groove of the plexiglass base 705, and then the silicone gasket 704 Put it on the lower plate electrode 703, then put the upper plate electrode 702 on the silica gel gasket, put the plexiglass cover 701 on the top, and fix the upper plate electrode 702 in the groove of the plexiglass cover 701. In order to facilitate the connection of the plate electrodes with the electrode wires, the upper plate electrode 702 and the lower plate electrode 703 respectively protrude from the cover 701 and the base 705 on both sides of the plate flow chamber 7 . Finally, the plexig...

Embodiment 3

[0058] Example 3 Cardiomyocyte Culture of Shear Force-Uniform Electric Field Combined Stimulation

[0059] Under sterile conditions, the cardiomyocytes of 1-3 day old SD rat suckling mice were divided into 1×10 5 / cm 2 The density is seeded on the lower plate electrode 703, and the lower plate electrode is made of conductive glass such as tin dioxide doped with fluorine. After pre-cultivation for 3 days, the lower plate electrode 703 inoculated with cardiomyocytes is fixed on the groove of the polycarbonate base 705, and then Put the silicone gasket 704 on the lower plate electrode 703, then place the upper plate electrode 702 on the silicone gasket, put the polycarbonate cover 701 on the top, and fix the upper plate electrode 702 in the groove of the polycarbonate cover 701 . In order to facilitate the connection of the plate electrodes with the electrode wires, the upper plate electrode 702 and the lower plate electrode 703 respectively protrude from the cover 701 and th...

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Abstract

The invention provides a cell culture device with shear force-uniform electric field stimulation, which comprises an animal cell culture box, a peristaltic pump, an upstream liquid storage bottle, a downstream liquid storage bottle, a signal generator and a flat-panel flow chamber, wherein the peristaltic pump and the upstream liquid storage bottle are connected with the downstream liquid storage bottle through a silicone tube to form a culture solution perfusion system which is used for loading shear force stimulation for cells; the signal generator is used for providing an electric signal for the flat-panel flow chamber, and the flat-panel flow chamber loads uniform electric field stimulation for the cells through a flat panel electrode-flat panel electrode system; and the flat-panel flow chamber is used for providing a base needed by cell growth and is connected with the culture solution perfusion system and the signal generator to provide a collectively-acted environment of shear force-uniform electric fields for the cells on flat panel electrodes. The cell culture device establishes a cell culture environment under shear force stimulation combined with uniform electric field stimulation, so that the defect that the prior art cannot simulate endothelial cells being in the collectively-acted environment of force-electrical stimulation is overcome, and a foundation of promoting the functional culture of cells and tissues is laid.

Description

technical field [0001] The invention relates to the fields of cell engineering and tissue engineering, in particular to a cell culture device that combines a mechanical environment and a uniform electric field environment. Background technique [0002] In tissue engineering, the in vitro functional culture of cells and tissues has become the core of tissue engineering and the necessary technical basis for the formation of tissue engineering industry. It is very important for the three-dimensional culture and functionalization of cells and tissues to provide an in vitro growth environment that is similar to the living conditions of cells in vivo or as favorable as possible. Among them, physical influence factors such as mechanical force and electric field play a role that cannot be ignored. [0003] The mechanical environment has an important impact on life activities at all levels of organs, tissues, cells and even sub-cells. For example, the shear force generated by the b...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/42
CPCC12M35/02C12M29/10C12M29/02
Inventor 樊瑜波李萍宋崴刘李珍贾璐刘成国
Owner BEIHANG UNIV
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