Breeding method for inducing parthenogenesis of corn by trifluralin

Abstract

The invention discloses a breeding method for inducing parthenogenesis of corn by trifluralin. The method is characterized by comprising the following steps of: selecting 48 percent trifluralin missible oil as an inducer, and diluting the trifluralin missible oil into 200-1,000-fold solution by using water; inducing, namely spraying or coating trifluralin diluent on thrums after the thrums on clusters come out; harvesting to obtain diploid seeds with homozygous genotype when seeds are mature; seeding in the next season, identifying truth according to phenotype and weeding out inferior plants, namely seeding the harvested clusters for the next generation, selectively harvesting rows according with a breeding goal, and thus obtaining a homozygous self-bred line of the corn. The invention has the advantages that: the trifluralin has low toxicity, can be used in the field under open conditions, and is safe to people, livestock and the environment; the inductivity is about 1.0 to 3.0 percent, pollination is not required, and the method is simple, easy to operate, low in cost and high in efficiency and can be used in a large scale; the diploid seeds are directly formed, and the process of doubling chromosomes is not required; and moreover, compared with that of the conventional seed selection method and a Stock 6 genetic induction method, the seed selection period is shortened by more than six generations and is shortened by more than one generation.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, in particular to a breeding method for inducing corn parthenogenesis by using trifluralin. Background technique [0002] In the conventional method of breeding maize inbred lines, at least 7 selfing generations are required to complete the breeding process of an inbred line, and more generations are required for materials with complex genetic backgrounds. In order to shorten the breeding cycle, existing methods both at home and abroad are to use the Stock 6 induction line to carry out genetic induction, and after inducing the production of haploid seeds, they are doubled into diploids with chromosome doubling agents such as colchicine; Chemical reagents such as colchicine treat filaments to induce maize to directly produce homozygous diploid seeds through parthenogenesis. However, these methods have the following disadvantages: [0003] 1. After Stock 6 genetic induction method, each se...

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Problems solved by technology

The workload is heavy and boring, and it is difficult to realize mechanization

[0004] 2. Some induced pairs of Stock 6 also have the problem of poor color rendering, so it is difficult to identify

[0005] 3. Most of the seeds induced by the Stock 6 genetic induction method are haploid, and they must undergo chromosome doubling to become normal plants. However, the chromosome doubling technique is cumbersome and the doubling rate is low

[0007]5. Using colchicine to induce parthenogenesis, the reagent is highly carcinogenic, the cost of the reagent is high, and it poses a great threat to human health and environmental safety. It must be strictly operated and controlled, and it is difficult to use it on a large scale in breeding.