Method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves

Abstract

The invention relates to a method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves after removing leaves. The method comprises the following steps of: taking 36-48 plants of lolium multiflorum which are consistent in growth and 3 years old, culturing for 7 weeks under the conditions that the temperature is 18-25 DEG C and the illumination time is 6-8 hours every day, respectively detecting the average carbon storage Cr in the root parts of the lolium multiflorum before removing the leaves, the average carbon storage Cs in stubbles of the lolium multiflorum before removing the leaves and the total average carbon storage Cn in regenerated leaf blades of the lolium multiflorum, and calculating the relative maximum amount RI of the stored carbohydrates capable of being utilized for regeneration of the lolium multiflorum leaves. According to the method for measuring the stored carbohydrates capable of being utilized for regenerationof the lolium multiflorum leaves, provided by the invention, the measuring method is simple, the relative maximum amount of the stored carbohydrates which can be utilized for regeneration of the leafblades of the lolium multiflorum before removing the leaves can be detected, the process is simple, the operation is simple, and the production efficiency and the working benefits can be further improved.

Description

technical field [0001] The invention relates to a measurement method, in particular to a method for measuring carbohydrates stored in regeneration and utilization of ryegrass leaves. Background technique [0002] Grass often exhibits "regeneration" after being eaten by herbivores or artificially cut, which is one of the most basic biological characteristics of pastures to maintain their own survival and resist damage from herbivores. Quantity is also important. Grass regeneration is a multifaceted and complicated problem, but many regeneration problems must face a basic fact, that is, the source of carbohydrates needed for regeneration after defoliation. Because the essential problem of pasture re-growth is a process of using energy and matter to produce new organic matter, however, defoliation of pasture will not only seriously inhibit its photosynthesis, reduce the supply of photosynthetic products, even in the case of severe defoliation, it will cause leading to a comp...

AI Technical Summary

Problems solved by technology

Grass regeneration is a multifaceted and complicated problem, but many regeneration problems must face a basic fact, that is, the source of carbohydrates required for regeneration after defoliation

Because the essential problem of pasture re-growth is a process of using energy and matter to produce new organic matter, however, defoliation of pasture will not only seriously inhibit its photosynthesis, reduce the supply of photosynthetic products, even in the case of severe defoliation, it will cause complete interruption of light action

[0003] However, at present, many scholars' research on the utilization of carbohydrates by pastures is limited to clarifying its beneficial effects on regeneration from a qualitative aspect, or using isotope techniques to verify that some of the carbohydrates that can be used for regeneration come from the stored carbohydrates before leaf removal. carbohydrate

It is of great significance to study the regeneration of pastures to find out how much carbohydrates stored before defoliation of pastures will directly participate in the growth of regenerated leaves. However, there have been no reports on specific measurement methods for this aspect so far.