Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying cytoplasm type of cytoplasmic-genic male sterile rice by detecting mitochondrial DNA

A mitochondrial and cytoplasmic technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of no cytoplasmic marker traits, no cytoplasmic distinction, etc.

Inactive Publication Date: 2012-07-18
HUNAN NORMAL UNIVERSITY
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since these cytoplasms have no cytoplasmic marker traits except those related to male sterility, it is impossible to distinguish their cytoplasms by common biological traits

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying cytoplasm type of cytoplasmic-genic male sterile rice by detecting mitochondrial DNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] In the present invention, 32 typical indica rice and typical japonica rice cytoplasms were used as controls, and the mitochondrial DNA sequences of 17 three-line sterile lines from different sources were analyzed, and it was found that different types of sterile cytoplasms had characteristic bases or base sequences, and the mitochondrial primers The amplified sequence difference of mt1, mt6, mt7 and mt11 established three mitochondrial DNA molecular fingerprints of wild type, red lotus type and BT type.

[0011] Using conventional rice Nipponbare as a control, PCR amplification products of mitochondrial DNA of three-line male sterile lines such as V20A were cloned and sequenced, and the sequence results are shown in Table 1.

[0012] Table 1

[0013]

[0014] Note: "-" indicates that the sequence is missing.

[0015] Mitochondrial primer

[0016] mt1: forward sequence 5-tcttcttcggacttgatgcac-3, reverse sequence 5-gcgcccttgaaatcatctta-3;

[0017] mt6: forward seque...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying cytoplasm type of cytoplasmic-genic male sterile rice by detecting mitochondrial DNA. The method comprises the following steps of: extracting the mitochondrial DNA from rice leaves serving as materials; performing polymerase chain reaction (PCR) amplification by using mitochondrial primers mt1, mt6, mt7 and mt11, and cloning and sequencing amplification products; and comparing with molecular fingerprints of the mitochondrial DNA of the cytoplasmic-genic male sterile rice to easily and efficiently identify the cytoplasm type of the cytoplasmic-genic male sterile rice.

Description

technical field [0001] The invention relates to a method for identifying the cytoplasm type of three-line sterile rice. Background technique [0002] In 1966, Japanese scholar Shinjo Changtomo bred a BT-type male sterile line (selected with Indian indica rice variety Chinsuran Boro II as cytoplasmic donor and japonica rice variety as nuclear donor) controlled by both cytoplasm and nucleus. In 1970, Yuan Longping, Li Bihu and others discovered a male sterile plant in Hainan wild rice, and bred it into a "wild defeat" type sterile line through nuclear replacement. Subsequently, the Honglian CMS line was bred by crossing Hainan Hongmans wild rice and indica variety Liantangzao. Later, the K-type, Gang-type, Yinshui-type and so on were bred. Since these cytoplasms have no cytoplasmic marker traits except those related to male sterility, it is impossible to distinguish their cytoplasms by common biological traits. Contents of the invention [0003] The object of the prese...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈良碧梁满中戴小军栾霁
Owner HUNAN NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com