Microcarrier cell reactor and ventilating pipe thereof

Abstract

The invention discloses a ventilating pipe for a microcarrier cell reactor, comprising a central ventilating pipe (1), wherein the bottom end of the central ventilating pipe (1) is provided with an air outlet hole (11); the central ventilating pipe (1) is externally provided with a sleeve (2); the sleeve (2) comprises a ventilating network pipe (21) and a defoaming network pipe (23) arranged above the ventilating network pipe (21); a connecting pipe (22) is arranged between the ventilating network pipe (21) and the defoaming network pipe (23); and the cross-section area of each mesh of the ventilating network pipe (21) is smaller than that of each microcarrier in the microcarrier cell reactor, and is smaller than that of the air outlet hole (11). The ventilating pipe disclosed by the invention has the advantages that not only can the oxygen supply need of cell culture be met, but also the damage caused to cells in the ventilating process can be avoided, and the survival rate of cell culture is increased. In addition, the invention also provides the microcarrier cell reactor comprising the ventilating pipe, and the microcarrier cell reactor has the same effect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a ventilation tube for a microcarrier cell reactor. In addition, the present invention also relates to a microcarrier cell reactor comprising the above-mentioned ventilation tube. Background technique [0002] Mammalian cell culture refers to the technique of maintaining cell growth and proliferation with culture medium under in vitro conditions of cells. A microcarrier cell reactor usually consists of a culture tank and a vent tube. The culture tank is filled with culture fluid and cells to provide a growth environment for the cells. The vent tube is used to deliver gas to the culture tank. During the culture process, the cells absorb the nutrients in the culture fluid and the dissolved oxygen in the gas for their own growth. need. [0003] There are mainly two methods for culturing and aeration of animal cell microcarriers at present. One is to use surface oxygen supply and sti...

AI Technical Summary

Problems solved by technology

One is to use the method of surface oxygen supply and stirring. The disadvantage of this method is that the gas-liquid specific surface area is too small, and the oxygen supply for surface ventilation is often insufficient.

Another method is to use deep aeration, that is, a ventilation tube is inserted into the bottom of the culture tank to supply air. The oxygen supply of this method can meet the needs of cell growth, but because the bubbles will trap cells during the rising process, so When the bubbles burst during ascent, the shear force generated by the rupture will tear the cells apart and cause cell death, which will cause a large number of cells to be damaged throughout the culture cycle

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