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Chicken embryo cultivation method of Trionyx sinensis systemic sepsis spherical virus

A spherovirus, chicken embryo culture technology, applied in the directions of microorganism-based methods, viruses/phages, biochemical equipment and methods, etc., can solve the problem of not culturing soft-shelled turtle systemic sepsis spherovirus and other problems, achieve high scientific research value and simple operation , to obtain convenient effects

Inactive Publication Date: 2013-10-02
NINGBO UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there are currently reports on chicken embryo culture of rare aquatic animal viruses at home and abroad, and there is no relevant research report on the method of cultivating soft-shelled turtle systemic septicemia spheroid virus using chicken embryo culture methods

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  • Chicken embryo cultivation method of Trionyx sinensis systemic sepsis spherical virus
  • Chicken embryo cultivation method of Trionyx sinensis systemic sepsis spherical virus

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Embodiment Construction

[0024] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0025] The following examples further illustrate the present invention, but the protection scope of the present invention is not limited thereto, and the protection scope is as the criterion.

[0026] The invention discloses a method for culturing chicken embryos of soft-shelled turtle systemic septicemia spheroid virus. SPF chicken embryos are used as the in vitro culture system of soft-shelled turtle systemic septicemia spheroid virus. Healthy chicken embryos that have been cultured for 4 to 6 days in advance are selected, and the pre-prepared chicken embryos are selected. The soft-shelled turtle systemic sepsis spheroidal virus inoculum was inoculated into the yolk sac of chicken embryos, and the culture was continued until the chicken embryos died. The strain of septicemia spheroid virus is P1015 strain, and the preservation number is CGMC...

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Abstract

The invention discloses a chicken embryo cultivation method of Trionyx sinensis systemic sepsis spherical virus. The method is characterized in that an SPF chicken embryo is used as an in vitro culture system of Trionyx sinensis systemic sepsis spherical virus; a healthy chicken embryo which has been pre-cultured for 4 to 6 days is selected; a pre-prepared Trionyx sinensis systemic sepsis spherical virus inoculation fluid is innoculated to the chick embryo yolk sac; cultivation is continued until the death of the chick embryo; and the dead chick embryo is collected to carry out homogenate centrifugal separation and obtain a supernatant containing Trionyx sinensis systemic sepsis spherical virus. It is the first time to apply chick embryo culture technique into the cultivation of Trionyx sinensis systemic sepsis spherical virus. In addition, the method can accomplish fast and efficient in vitro subculturing of Trionyx sinensis systemic sepsis spherical virus and is stable and convenient.

Description

technical field [0001] The invention relates to an in vitro culture method of soft-shelled turtle systemic septicemia spheroid virus, in particular to a chicken embryo culture method of soft-shelled turtle systemic septicemia spheroid virus. Background technique [0002] Turtle systemic septicemia spheroid virus is a new type of virus isolated from diseased soft-shelled turtles. The virus can cause systemic hemorrhage and septicemia in soft-shelled turtles, and the cumulative mortality rate can reach 90-100% within 7-10 days. Since the research on the virus is in the initial stage, the materials are all from the diseased soft-shelled turtle, and the supply is unstable and very limited, which brings great inconvenience to the research on the virus and the prevention and treatment of the diseases it causes. In view of this, whether to establish a method suitable for the large-scale cultivation of soft-shelled turtle systemic septicemia spheroid virus and provide stable materi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12N7/02C12R1/93
Inventor 李登峰周永强刘联国彭娇陈炯
Owner NINGBO UNIV
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