Method for removing impurities from gentamicin
A technology of gentamicin and concentrated liquid, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of products failing to meet the requirements of the new pharmacopoeia, low yields, etc., and alleviate the shortage of supply. Pressure, the effect of improving product quality
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Embodiment 1
[0013] A kind of gentamicin impurity removal method of the present invention adopts following steps to carry out impurity removal:
[0014] Take 200,000 u / ml of gentamicin concentrate (from Nanyang Pukang Pharmaceutical Co., Ltd.), and dilute it to 60,000-80,000 u / ml with purified water, so that the volume of the sample will not be too large for subsequent concentration The treatment brings more pressure, and it will not cause uneven adsorption and slow adsorption speed due to high sample concentration, then adjust the pH to 6.0-8.0 with commercially available concentrated hydrochloric acid, and slowly adsorb on the weak acidic cation exchange resin HZD -2 (purchased from Shanghai Huazhen Technology Co., Ltd., East China University of Science and Technology), the adsorption flow rate is 0.5-0.8 BV / h. After the adsorption is completed, wash with about 2BV of purified water until impurities such as chloride ions are removed, and then wash with 0.05-0.15 mol / L dilute ammonia wate...
Embodiment 2
[0017] A kind of gentamicin impurity removal method of the present invention adopts following steps to carry out impurity removal:
[0018] Take 200,000 u / ml of gentamicin concentrate (from Nanyang Pukang Pharmaceutical Co., Ltd.), dilute it to 60,000-80,000 u / ml with purified water, and then adjust the pH to 6.0-8.0 with commercially available concentrated hydrochloric acid. Slowly adsorbed on the weakly acidic cation exchange resin HZD-2 (purchased from Shanghai Huazhen Technology Co., Ltd., East China University of Science and Technology), and the adsorption flow rate was 0.5-0.8 BV / h. After the adsorption is completed, wash with 3.0BV purified water to remove impurities such as chloride ions, and then wash with 0.05-0.15 mol / L dilute ammonia water, with an amount of about 10-15 BV and a flow rate of 1.0-1.5 BV / h to remove most of the Small components with poor adsorption to resin. When the effluent unit is 1700 u / ml, stop washing. Continue to wash with 0.3-0.6 mol / L ammo...
Embodiment 3
[0021] A kind of gentamicin impurity removal method of the present invention adopts following steps to carry out impurity removal:
[0022] Take 200,000 u / ml of gentamicin concentrate (from Nanyang Pukang Pharmaceutical Co., Ltd.), dilute it to 60,000-80,000 u / ml with purified water, and then adjust the pH to between 6.0-8.0 with commercially available concentrated hydrochloric acid. Slowly adsorbed on the weakly acidic cation exchange resin HZD-2 (purchased from Shanghai Huazhen Technology Co., Ltd., East China University of Science and Technology), and the adsorption flow rate was 0.5-0.8 BV / h. After the adsorption is completed, wash with 3.0BV purified water to remove impurities such as chloride ions, and then wash with 0.05-0.15 mol / L dilute ammonia water, with an amount of about 10-15 BV and a flow rate of 1.0-1.5 BV / h to remove most of the Small components with poor adsorption to resin. When the effluent unit is 1800 u / ml, stop washing. Continue to wash with 0.3-0.6 mo...
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