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Living body measurement method for body length of fish fry

A measurement method and larvae technology, applied in the direction of measuring devices, instruments, optical devices, etc., to achieve the effects of reducing mechanical damage, loose measurement time, and accurate values

Inactive Publication Date: 2012-08-15
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problem that the existing method for measuring the body length of larvae cannot simultaneously satisfy the problem of keeping the fish alive and quickly measuring multiple samples, and provides a method for measuring the body length of larvae in living body

Method used

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  • Living body measurement method for body length of fish fry
  • Living body measurement method for body length of fish fry
  • Living body measurement method for body length of fish fry

Examples

Experimental program
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specific Embodiment approach 1

[0009] Specific embodiment one: the method for measuring the length and living body of larvae in this embodiment is carried out according to the following steps: 1. Put the larvae to be tested into a transparent culture dish filled with water, and put a ruler with a length of 10mm into the container filled with larvae. In a transparent petri dish; 2. The bottom of the petri dish is lined with a background to make the color of the larvae contrast with the background color; 3. Use a digital camera to take pictures of the entire petri dish, and use image processing software to measure the larvae in the photos respectively. Body length and scale length; 4. Use the formula L=10×b / a to calculate the real body length of the larvae through statistical software. In the formula, L is the real body length of the fry in mm; b is the body length of the larvae in the photo; a is the length of the scale in the photo , that is, to complete the live measurement of the body length of the larvae....

specific Embodiment approach 2

[0012] Embodiment 2: This embodiment differs from Embodiment 1 in that: the scale described in step 1 is on the same level as the larvae to be measured. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0013] Specific embodiment three: the present embodiment larvae body grows living body measurement method, carries out according to the following steps: one, 85 tail larvae to be measured are put into the transparent petri dish that the diameter that fills water is 80mm, and length is 10mm ruler Put it into a transparent petri dish filled with larvae, and the scale is on the same level as the larvae to be measured; 2. The bottom of the petri dish is lined with a white background, so that the color of the larvae is contrasted with the background color; 3. Take pictures with a digital camera For the photos of the whole petri dish, the photographs taken shall be measured by photoshop image processing software respectively for the body length and scale length of 30 normal larvae in the photo; 4. Calculate the real body length of larvae by using the formula L=10×b / a by Excel software , in the formula, L is the real body length of the fry in mm; b is the body length of the larvae in ...

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Abstract

The invention relates to a living body measurement method for body length of a fish fry. The method in the invention is mainly used for solving that the problem that the existing method for measuring the body lent of the fish fry can not meet the requirement that multiple samples can be rapidly measured while a fish body is guaranteed to be survived. The method comprises the following steps of: firstly, placing the fish fry to be measured into a culture dish filled with water, and placing a scale with the length of 10mm into the culture dish; secondly, lining a background at the bottom of the culture dish, thus contrast is formed between colour of the fish fry and the colour of the background; thirdly, shooting a picture of the whole culture dish by a digital camera, and respectively measuring the body length of the fish fry and the length of the scale in the picture by utilizing image processing software; and fourthly, calculating real body length of the fish fry by utilizing a scaling formula, thus living body measurement of the body length of the fish fry is completed. According to the method disclosed by the invention, body length measurement on the fish fry is carried out by virtue of a digital camera, survival rate of the fish fry is guaranteed, images of a large number of samples are stored in a short time, measurement time is more leisure and freer, and numerical measurements are more accurate; and the method disclosed by the invention is used for living body detection for the body length of the fish fry.

Description

technical field [0001] The invention relates to a method for measuring live body length of larvae. Background technique [0002] Body length measurement is a very important biological indicator in the study of fish growth characteristics. Because fish are slippery, swim fast, jump easily, and are difficult to control, they are generally anesthetized with drugs and measured with standard measuring tools such as tape measure, ruler, and vernier caliper. Larvae are fish fry that have hatched from the egg membrane until the yolk has been absorbed and have odd fin folds. Their tissues and organs are not fully developed, their bodies are weak, and their resistance to stress is poor. After the use of anesthesia, the fish will also die and bend. Therefore, there are currently two commonly used methods for measuring the body length of larvae: one is in vivo measurement, that is, measuring with a vernier caliper after the larvae are still in the water body. The advantage is that the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01B11/02
Inventor 徐伟耿龙武李池陶
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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