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Method for measuring pipemidic acid by using resonance scattering spectrum

A technique of resonance scattering and spectrometry, applied in the field of analytical chemistry, can solve problems such as difficult popularization, cumbersome operation process, and large interference, and achieve the effect of simple equipment, easy-to-obtain reagents, and high sensitivity

Inactive Publication Date: 2012-08-22
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the mercury used in the oscillometric titration method is a highly toxic substance, which is very harmful to the health of the operator, while the high performance liquid chromatography is difficult to popularize because of expensive instruments and cumbersome operation process; although the spectrophotometry method is simple to operate, but Low sensitivity, large interference
There is no report on the determination of pipemidic acid by resonance scattering spectrometry

Method used

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  • Method for measuring pipemidic acid by using resonance scattering spectrum
  • Method for measuring pipemidic acid by using resonance scattering spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] (1) Take five 5 mL graduated test tubes, pipette 300 μL pH 3.6 acetic acid-sodium acetate buffer solution, 400 μL 2.0 mmol / L sodium tetraphenylborate solution, and then add 10 μL, 50 μL, 200 μL , 400 μL, 950 μL pipemidic acid standard solution with a concentration of 200 μg / mL, mix well, let stand at room temperature for 20 minutes, dilute to 2.0 mL with twice distilled water, mix well;

[0018] (2) Take another 5 mL graduated test tube and use the method in step (1) to prepare the blank control system solution without adding pipemidic acid standard solution;

[0019] (3) Take an appropriate amount of pipemidic acid standard solution and blank control system solution prepared according to steps (1)-(2), put them in a 1cm cuvette, and set a photomultiplier tube on the Cary Eclipse fluorescence spectrophotometer The voltage is 450v, the incident slit and the excitation slit are both 2.5nm, and under the condition that the excitation wavelength is equal to the emission wav...

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Abstract

The invention discloses a method for measuring pipemidic acid by using a resonance scattering spectrum. Pipemidic acid and tetraphenylboron sodium react in acetic acid-acetic acid buffer solution with the pH value being 3.6 to form ionic associate particles, wherein the particles generate a resonance scattering spectrum peak at the wavelength being 480nm, and the resonance scattering intensity is linearly related to the concentration of the pipemidic acid so as to provide a new method for measuring the content of the pipemidic acid by using the resonance scattering spectrum. In comparison with the conventional method, the method provided by the invention has the advantages of simple instrument, convenience in operation, rapidness, easily obtained reagent and high sensitivity.

Description

technical field [0001] The invention relates to analytical chemistry, in particular to a method for measuring pipemidic acid by resonance scattering spectroscopy. Background technique [0002] The chemical name of pipemidic acid is 8-ethyl-5-oxo-5,8-dihydro-2(1-piperazinyl)-(2,3-d)pyrimidine-6-carboxylic acid, PPA, referred to as PPA, is a pyridone acid antibacterial drug, which has the characteristics of broad antibacterial spectrum, strong activity, and low toxicity. It is a commonly used drug for the treatment of intestinal diseases. Therefore, it is of great significance to monitor the quality of pipemidic acid. At present, the methods for the determination of pipemidic acid mainly include spectrophotometry, voltammetry, oscillographic polarography, titration, chemiluminescence, fluorescence spectroscopy, high performance liquid chromatography and so on. Most of these methods have a number of significant disadvantages. For example, the mercury used in the oscillometri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/63
Inventor 蒋治良胡茂辉梁爱惠
Owner GUANGXI NORMAL UNIV
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