Preparation method for detection reagent box of sore mouth disease antibody

A technology for antibody detection and kits, which is applied in the field of preparation of aphthous ulcer antibody detection kits, can solve the problems that the prevalence of aphthous ulcers cannot be explained, cannot be widely promoted, and the operation is complicated, so as to prevent false positive results and achieve good results. Detection, high sensitivity effect

Active Publication Date: 2012-09-19
NORTHWEST A & F UNIV
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Problems solved by technology

There are many detection methods at present, including virus isolation and identification, PCR detection, LAMP detection, immunoelectron microscope analysis, serological detection, etc. These methods have the disadvantages of complicated operation, high cost, and inability to be widely promoted. The situation cannot be explained, and the detection can only be carried out after the animal is infected with the oral ulcer virus, and the animal that has just been infected with the oral ulcer virus and has not yet developed symptoms or has formed a recessive infection cannot be detected

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  • Preparation method for detection reagent box of sore mouth disease antibody
  • Preparation method for detection reagent box of sore mouth disease antibody
  • Preparation method for detection reagent box of sore mouth disease antibody

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preparation example Construction

[0014] The preparation of the oral ulcer antibody detection kit includes the following steps: 1) isolation and preliminary culture of oral ulcer virus; 2) cloning and prokaryotic expression of the early expression protein genes B2L, F1L, VIR, and CBP of oral ulcer virus; 3) prokaryotic expression Protein and oral ulcer virus cell culture fluid are respectively used as coating antigens to detect the serum samples to be tested, and the detection effect of which protein is determined by comparison, so as to determine the best coating antigen, as a detection kit, to determine its infection status and virus popular features. (4) The establishment of a mouth ulcer antibody detection kit and the specificity, sensitivity and repeatability tests.

[0015] Among them, the selected gene is the early expression protein gene of the aphthus virus, which is unique to the aphthus virus, and begins to be expressed in the early stage of infection, and the similarity between different strains is...

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Abstract

The invention relates to a preparation method for a detection reagent box of a sore mouth disease antibody. According to the method, primary cells of testicles of calves are used, a sixth generation of cell culture fluid obtained through continuous subculturing of sore mouth disease viruses serves as a template for prokaryotic clone, soluble expressed protein is obtained through prokaryotic expression and the detection reagent box of the sore mouth disease antibody is prepared through the expressed protein serving as a coating antigen. Evidences are provided for epidemiological survey of the sore mouth disease according to the analysis and determination of epidemiological characteristics of the sore mouth disease.

Description

Technical field: [0001] The invention relates to a preparation method of a mouth ulcer antibody detection kit. Background technique: [0002] Sheep contagious impetigo, commonly known as Sore mouth disease, is an acute, contact and epitheliophilic zoonotic disease caused by Sore mouth virus infection, mainly infecting sheep and goats, World Organization for Animal Health (OIE) listed the disease as a notifiable animal disease, and my country listed it as a first-class animal disease. The disease exists and occurs in almost all sheep-raising countries and regions in the world. Under natural conditions, it mainly affects sheep and goats, and goats are more frequent. The disease is often popular in groups, and lambs aged 3-6 months are most susceptible to infection, and adult sheep are less likely to be infected. Sick sheep and infected sheep are the source of infection of the disease. The virus can be excreted with the saliva, pustules and blister secretions of the sick sheep...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
Inventor 陈德坤李杰李前瑞田婷婷姚运亮许君艳罗军曹斌云
Owner NORTHWEST A & F UNIV
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