Immune-fluorescence test strip component for rapidly detecting C-reactive protein quantitatively, detection card component produced by same and method for preparing same

Abstract

The invention discloses an immune-fluorescence test strip component for rapidly detecting C-reactive protein quantitatively, a detection card component produced by the same and a method for preparing the same. The test strip component comprises a test strip and a platinum porphyrin marked specific antibody independently packed. The test strip comprises a bottom lining, a water absorption pad, coating analysis film and a sample pad. The coating analysis film is provided with a detection line and a quality control line, a specific antibody coated by the detection line is a C-reactive protein resistance monoclonal antibody, and a specific antibody coated by the quality control line is a rabbit intravenous gamma globulin (IgG) antibody; the detection card component comprises a test strip, a card box composed of a cover plate and a back plate and a latinum porphyrin marked specific antibody independently packed. The test strip component for detecting C-reactive protein in body fluid has the advantages of being simple in operation, rapid, sensitive, good in specificity and the like, and having good clinical application prospect.

Description

technical field [0001] The invention belongs to the field of medical testing, and in particular relates to an immunofluorescence test strip component for rapid quantitative detection of C-reactive protein and a preparation method thereof, and a detection card component supported by the immunofluorescence test strip component and a preparation method thereof. Background technique [0002] C-reactive protein (C-reactive protein, CRP) is a ring-shaped pentaspherical protein, belonging to Oligomeric calcium-binding protein, with a relative molecular mass of about 120,000. It is composed of five identical monomers with non-covalent bonds. It is an inflammatory lymphokine Interleukin-6, interleukin-1, and tumor necrosis factor stimulate the synthesis of liver epithelial cells. Since C-reactive protein begins to increase 6-8 hours after infection, reaches a peak at 24-48 hours, and the peak value can reach hundreds of times the normal level. After the infection is eliminated, its c...

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Problems solved by technology

[0006] And the Chinese patent No. 102087293 discloses an immunochromatographic test paper and a preparation method thereof for quantitatively detecting C-reactive protein throughout the whole process. Although it adopts a fluorescent quantitative technology, it does not have a clear and specific fluorescent material; at the same time, it uses fluorescent latex Drying and fixing the particles on the binding pad will definitely affect their dissolution and release speed on the glass cellulose membrane, as well as their crawling speed on the nitrocellulose membrane, and the entire detection time should far exceed the required 10-second measurement time

At the same time, it is difficult to form an effective antigen-antibody reaction within 10 seconds for the C-reactive protein in the test object and the C-reactive protein antibody labeled with fluorescent latex particles, as well as the C-reactive protein antibody immobilized on the nitrocellulose membrane. , the immunological response is insufficient, which will inevitably lead to low detection sensitivity and narrow detection linear range, which is difficult to meet the needs of clinical detection

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