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Low-temperature aerobic denitrifying strain Pseudomonas psychrophila Den-03 and screening method and application thereof

A technology of aerobic denitrification bacteria and aerobic denitrification, applied in the direction of chemical instruments and methods, biochemical equipment and methods, methods based on microorganisms, etc., to achieve the effects of reducing production energy consumption, simple activation, and improving nitrogen conversion rate

Active Publication Date: 2012-09-26
江苏哈宜环保研究院有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The main body of aerobic denitrification application is the aerobic denitrification bacteria group. So far, aerobic denitrification bacteria are mainly concentrated in Pseudomonas, Alcaligenes and Paracoccus. and Bacillus (Bacillus), there are many studies on the application and mechanism of strains, but there are fewer reports on low-temperature aerobic denitrifying bacteria

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  • Low-temperature aerobic denitrifying strain Pseudomonas psychrophila Den-03 and screening method and application thereof
  • Low-temperature aerobic denitrifying strain Pseudomonas psychrophila Den-03 and screening method and application thereof
  • Low-temperature aerobic denitrifying strain Pseudomonas psychrophila Den-03 and screening method and application thereof

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specific Embodiment approach 1

[0025] Specific implementation mode 1: This implementation mode provides screening steps for aerobic denitrifying bacteria

[0026] 1. In winter, take 10mL of fresh activated sludge from the Harbin Municipal Sewage Plant and add it to a 100mL aerobic denitrification medium in a triangular flask, and place it at a temperature of 10 oC. Cultivate for 48 hours in a shaker with a rotating speed of 150r / min to obtain the enriched bacterial solution; 2. Take 10mL of the enriched bacterial solution and add it to a 100mL aerobic denitrification medium conical flask, repeat the culture conditions of step 1 to obtain the domesticated bacteria 3. Take 100 μL of domesticated bacteria solution and add it to the solid aerobic denitrification medium plate, spread it evenly by coating method, and place it at a temperature of 10 o In the incubator of C, cultivate for 48-72 hours to obtain a single colony; 4. Repeat the operation step 3 3-4 times to complete the acquisition of low-temperature a...

specific Embodiment approach 2

[0030] Specific embodiment two: take the single colony of low-temperature aerobic denitrifying bacteria obtained in specific embodiment one and culture it in low-temperature aerobic denitrification medium equipped with sterilized aerobic denitrification medium for 96 hours, and the rotating speed is 150r / min, which is low-temperature aerobic Growth and degradation curves of denitrifying bacteria. The result is as figure 1 Shown, 96h aerobic denitrifier OD 600 tends to be stable at around 1. The pollutant degradation rate reached 70%, indicating that the aerobic denitrifying bacteria obtained in this embodiment have good degradation ability.

specific Embodiment approach 3

[0031] Embodiment 3: The difference from Embodiment 2 is that the aerobic denitrifying bacteria liquid after 96 hours of cultivation is placed in the SBR reactor. According to four different nitrate nitrogen concentration 100mg / L, 80mg / L, 60mg / L, 40mg / L respectively pack in four conical flask SBR reactors, add 10% activated sludge in each 500mL conical flask, 10 o C culture, 150 r / min, aerobic culture for 10 days. Reaction cycle: a cycle of 12 hours, water intake 0.5 hours, aeration 8 hours, sedimentation 2 hours, drainage and idle time 1.5 hours. The result is as figure 2 As shown, with the prolongation of the culture time, the degradation effect of different concentrations of nitrate nitrogen was obvious in 8 days, and the concentration of nitrate nitrogen all reached below 20mg / L, indicating that the low-temperature aerobic denitrification bacteria used in this implementation have good denitrification ability.

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Abstract

The invention belongs to the technical field of biological engineering and environmental engineering and discloses a low-temperature aerobic denitrifying strain Pseudomonas psychrophila Den-03. The strain is suitable for growing in a neutral culture medium at the pH (potential of hydrogen) of 7.2 and at the temperature of 10 DEG C, and is characterized in that the strain is atrichous, aerobic and capable of achieving denitrification, has yellowish-white cells and can grow with nitrate nitrogen serving as energy, and both catalase and oxidase are positive. The strain obtained by screening solves the problems of low production efficiency, low denitrification efficiency in winter and the like during sewage treatment of northern cold cities, removal rate of TN (total nitrogen) in low-temperature domestic sewage reaches 74%, and on the premise of guaranteeing stable denitrification efficiency, denitrification rate of urban sewage treatment systems in winter is increased, production efficiency is improved, production energy consumption is reduced, and the stain is of great significance to realization of short-range synchronous nitrification and denitrification.

Description

technical field [0001] The invention belongs to the technical fields of biological engineering and environmental engineering, and relates to the screening of bacterial strain Pseudomonas psychrophila Den-03 and its application in the denitrification process of low-temperature sewage. Background technique [0002] In the 21st century, the global water resource crisis has brought great troubles to human beings, especially as a developing country with rapid economic development, accelerating water pollution control has become one of the main goals of strengthening water resource protection in my country. The accumulation of various forms of nitrogen such as ammonia nitrogen in concentrated urban domestic sewage and nitrate nitrogen produced by a large amount of chemical fertilizers has exacerbated the degree of eutrophication of lake water, affecting and restricting social and economic development. During the "Twelfth Five-Year Plan" period, in the existing and newly-built sewa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/38C02F101/16
Inventor 马放崔迪李昂庞长泷邱天蔡蕊张斯孔祥震
Owner 江苏哈宜环保研究院有限公司
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