Bovine semen protective agent
A protective agent and semen technology, which is applied in the field of cattle reproduction, can solve the problems of decreased sperm motility, inability to sex-controlled separation by flow cytometry, and inability to freeze semen for storage and treatment, and achieve the effect of prolonging the vitality maintenance period and facilitating long-distance transportation
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Embodiment 1
[0025] Example 1 Preparation of Bovine Semen Protective Agent 562
[0026] Formulation of table 5 protective agent
[0027]
[0028] Table 3 The formula of mother liquor 1
[0029]
[0030]
[0031] pH value 6.8, prepared with water;
[0032] Table 4 The formula of mother liquor 2
[0033]
[0034] pH value 7.4, prepared with water;
[0035] Among them, mother liquor 1 and mother liquor 2 were pre-prepared and stored at 4°C; fresh egg yolk liquid was added within 24 hours before use.
Embodiment 2
[0036] The preparation of embodiment 2 bovine semen protective agent
[0037] Formulation of table 5 protective agent
[0038]
[0039] Table 6 The formula of mother liquor 1
[0040]
[0041] pH value 6.8, prepared with water;
[0042] Table 7 The formula of mother liquor 2
[0043]
[0044] pH value 7.4, prepared with water;
[0045] Among them, mother liquor 1 and mother liquor 2 were pre-prepared and stored at 4°C; fresh egg yolk liquid was added within 24 hours before use.
Embodiment 3
[0046] The preparation of embodiment 3 bovine semen protective agent
[0047] Formulation of table 5 protective agent
[0048]
[0049] Table 8 The formula of mother liquor 1
[0050]
[0051] pH value 6.8, prepared with water;
[0052] Table 9 The formula of mother liquor 2
[0053]
[0054]
[0055] pH value 7.4, prepared with water;
[0056] Among them, mother liquor 1 and mother liquor 2 were pre-prepared and stored at 4°C; fresh egg yolk liquid was added within 24 hours before use.
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