Bovine semen protective agent
A protective agent and semen technology, which is applied in the field of cattle reproduction, can solve the problems of inability to separate sex by flow cytometry, decline in sperm motility, and inability to freeze semen for storage and processing, and achieve the effect of facilitating long-distance transportation and prolonging the period of viability
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Embodiment 1
[0024] Example 1 Preparation of Bovine Semen Protective Agent 562
[0025] The formula of table 5 protective agent
[0026]
[0027] Formulation of mother liquor 1 of table 3
[0028]
[0029]
[0030] pH value 6.8, prepared with water;
[0031] Formulation of mother liquor 2 of table 4
[0032]
[0033] pH value 7.4, prepared with water;
[0034] Among them, mother liquor 1 and mother liquor 2 were pre-prepared and stored at 4°C; fresh egg yolk liquid was added within 24 hours before use.
Embodiment 2
[0035] The preparation of embodiment 2 bovine semen protective agent
[0036] The formula of table 5 protective agent
[0037]
[0038] Formulation of mother liquor 1 of table 6
[0039]
[0040] pH value 6.8, prepared with water;
[0041] Formulation of mother liquor 2 of table 7
[0042]
[0043] pH value 7.4, prepared with water;
[0044] Among them, mother liquor 1 and mother liquor 2 were pre-prepared and stored at 4°C; fresh egg yolk liquid was added within 24 hours before use.
Embodiment 3
[0045] The preparation of embodiment 3 bovine semen protective agent
[0046] The formula of table 5 protective agent
[0047]
[0048] Formulation of mother liquor 1 of table 8
[0049]
[0050] pH value 6.8, prepared with water;
[0051] The formula of table 9 mother liquor 2
[0052]
[0053]
[0054] pH value 7.4, prepared with water;
[0055] Among them, mother liquor 1 and mother liquor 2 were pre-prepared and stored at 4°C; fresh egg yolk liquid was added within 24 hours before use.
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