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Bovine semen protective agent

A protective agent and semen technology, which is applied in the field of cattle reproduction, can solve the problems of inability to separate sex by flow cytometry, decline in sperm motility, and inability to freeze semen for storage and processing, and achieve the effect of facilitating long-distance transportation and prolonging the period of viability

Active Publication Date: 2013-08-21
AIDE INSPECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after sperm collection is stored at room temperature for more than 8 hours, the sperm motility will decline rapidly, and conventional frozen semen storage can no longer be performed, nor can it be effectively used for sex control separation by flow cytometry

Method used

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  • Bovine semen protective agent
  • Bovine semen protective agent
  • Bovine semen protective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of Bovine Semen Protective Agent 562

[0025] The formula of table 5 protective agent

[0026]

[0027] Formulation of mother liquor 1 of table 3

[0028]

[0029]

[0030] pH value 6.8, prepared with water;

[0031] Formulation of mother liquor 2 of table 4

[0032]

[0033] pH value 7.4, prepared with water;

[0034] Among them, mother liquor 1 and mother liquor 2 were pre-prepared and stored at 4°C; fresh egg yolk liquid was added within 24 hours before use.

Embodiment 2

[0035] The preparation of embodiment 2 bovine semen protective agent

[0036] The formula of table 5 protective agent

[0037]

[0038] Formulation of mother liquor 1 of table 6

[0039]

[0040] pH value 6.8, prepared with water;

[0041] Formulation of mother liquor 2 of table 7

[0042]

[0043] pH value 7.4, prepared with water;

[0044] Among them, mother liquor 1 and mother liquor 2 were pre-prepared and stored at 4°C; fresh egg yolk liquid was added within 24 hours before use.

Embodiment 3

[0045] The preparation of embodiment 3 bovine semen protective agent

[0046] The formula of table 5 protective agent

[0047]

[0048] Formulation of mother liquor 1 of table 8

[0049]

[0050] pH value 6.8, prepared with water;

[0051] The formula of table 9 mother liquor 2

[0052]

[0053]

[0054] pH value 7.4, prepared with water;

[0055] Among them, mother liquor 1 and mother liquor 2 were pre-prepared and stored at 4°C; fresh egg yolk liquid was added within 24 hours before use.

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Abstract

The invention provides a protective agent for prolonging the vitality retention period of fresh bovine semen, which is prepared by mixing a mother liquor 1, a mother liquor 2 and fresh egg yolk liquid according to a certain proportion. Due to the adoption of the protective agent provided by the invention, the vitality of fresh bovine semen can still be maintained to be higher than 65% within 16 hours after semen collection, and the semen mortality of the fresh bovine semen is controlled to be 5% to 13% within 16 hours after semen collection. The results show that by using the bovine semen protective agent provided by the invention, the vitality retention period of the fresh bovine semen can be effectively prolonged, so that the long-distance transport is convenient, and a technical support and guarantee is provided for subsequent semen freezing treatment or sex-controlling separation treatment.

Description

technical field [0001] The invention relates to the technical field of cattle reproduction, in particular to a protective agent for prolonging the vigor maintenance period of fresh bovine semen. Background technique [0002] The newly collected bovine semen is generally stored at room temperature for less than 8 hours, and the semen can maintain sufficient vitality for subsequent frozen semen processing or sex control separation processing. However, after sperm collection is stored at room temperature for more than 8 hours, the sperm motility will decline rapidly, and conventional frozen semen storage can no longer be performed, nor can it be effectively used for sex control separation by flow cytometry. Therefore, how to realize the long-distance transportation of fresh semen has become an urgent technical problem to be solved when the place where the fresh semen is used is far from the place where the semen is collected. Contents of the invention [0003] The purpose of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 祝加贝
Owner AIDE INSPECTION TECH