Anti-tumor biological patch

A technology of biological patches and anti-tumor drugs, which is applied in the field of anti-tumor biological patches, can solve the problems of non-feasibility, increased recurrence of tumor cell implantation, etc., and achieve the effect of less side effects and increased curative effect of chemotherapy

Inactive Publication Date: 2012-10-03
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Bio-patch is the current clinical application trend and research hotspot. Bio-materials have the characteristics of easy growth of surrounding tissues and neovascularization, anti-adhesion, and degradability. Compared with other commonly used synthetic materials in clinical

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of anti-tumor biological patch

[0033] A. Methods of coating and dip-dyeing patches

[0034] A typical method of compositing a patch using a square 8cmx10cm is described below. For larger patches, a larger volume of polymer / drug solution is used.

[0035] Briefly, sufficient polymer was weighed directly into a 20ml glass scintillation vial and enough DCM was added to obtain a 2% w / v solution. The vial was then capped and mixed by hand to dissolve the polymer. The patch was then held in a vertical orientation using micro-clamps attached to a fixture 6-12 inches above the fume hood platform to enable horizontal spraying. Using an automatic pipette, transfer an appropriate volume (minimum 5ml) of the 2% polymer solution into a separate 20ml glass scintillation vial. An appropriate amount of nanoparticles of 5-fluorouracil combined with rapamycin was then added to the solution and mixed by hand shaking.

[0036] To prepare for nebulization, the cap of the ...

Embodiment 2

[0041] In vitro drug release rate

[0042] Small pieces (0.5x0.5 cm) of patches (n=4) coated with nanoparticles of 5-fluorouracil combined with rapamycin were placed in 14 ml glass tubes, followed by the addition of 10 ml phosphate containing 0.4 g / L albumin Buffered saline (PBS, pH=7.4). Tubes were incubated at 37°C with gentle swirl mixing at 8 rpm. At regular intervals, 10 ml of the supernatant was removed for analysis of 5-fluorouracil and rapamycin and replaced with fresh PBS / albumin buffer. 1 ml of dichloromethane was added to the removed supernatant and the tube was capped and shaken by hand for 1 minute to allow all released 5-fluorouracil and rapamycin to partition into the separate dichloromethane phase. The tubes were then centrifuged at 500 x g for 1 min, 10 ml of the upper aqueous phase was removed and discarded, and the dichloromethane phase was evaporated at 50 °C for 20 min under nitrogen. 1 ml of 60% acetonitrile in water (v / v) was added to each tube to dis...

Embodiment 3

[0044] In vivo supplement effect

[0045] General anesthesia was introduced into domestic pigs. Shave the abdominal area and disinfect the skin with a wash. Under aseptic conditions, a vertical incision was made on one side of the abdomen to expose the abdominal cavity. The synthetic mesh is repaired in the abdominal wall or replaces the peritoneum to contact the viscera of the abdominal cavity. Animals were randomly divided into 4 groups of 5 pigs each receiving synthetic patches coated with (1) carrier polymer only; Carrier polymer; (3) loading 5% 5-fluorouracil and rapamycin nanoparticle carrier polymer or (4) loading 10% 5-fluorouracil and rapamycin nanoparticle carrier polymer. Shut up.

[0046] A contralateral abdominal incision was made in the same manner and a control uncoated patch was used to repair the abdominal wall or replace the peritoneum. Let the animal recover.

[0047] Animals were sacrificed at 1 month, 3 months and 6 months after operation. Remove th...

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Abstract

The invention provides a patch for repairing tissue defects or wounds after tumor resection. The patch is characterized in that the patch is a biological patch, the biological patch can be coated or dipped by nanoscale anti-tumor drugs to prepare absorbable coatings which have controlled-release effects for chemotherapeutic drugs, and the patch which is anti-tumor and has the function of repairing tissue defects is prepared.

Description

【Technical field】 [0001] The invention relates to an anti-tumor biological patch, in particular to a composite patch used for repairing tissue defects after tumor resection. 【Background technique】 [0002] The definition of biological patch refers to the tissue taken from the same or different species, which is decellularized to remove various cells contained in the tissue while retaining the extracellular matrix (extracellular matrix, ECM) / acellular tissue matrix (acellular tissue matrix, The three-dimensional frame structure of ACTM) can be used to repair human soft tissue materials, or biological patches made of artificially synthesized biomaterials and their synthesis or composite materials with degradable materials. According to the tissue source, it can be divided into allogeneic materials such as dermal acellular matrix (acellular dermis matrix, ADM), amniotic membrane, dura mater or other collagenous matrix tissues. or other collagen matrix tissue, there are dozens ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/24A61L27/20A61L27/18A61L27/54
Inventor 申英末陈杰田茂霖
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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