Sub-totipotent stem cell, preparation method and application thereof
A technology of sub-totipotent stem cells and uses, applied in the field of sub-totipotent stem cells, the preparation of sub-totipotent stem cells, can solve the problems of poor understanding of stem cells
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Embodiment 1
[0073] Isolation of CD151 by Adherent Subculture + CD184 - Oct4 + subpluripotent stem cells
[0074] Cut off human placenta for CD151 + CD184 - Oct4 + Preparation of stem cell products. The specific steps are as follows: 1) Aseptically collect the cut human placenta; 2) Cut to small fragments 3 Small tissue pieces were digested with 0.5 mg / mL collagenase I at 37°C for 1 hour, and 1 mg / mL trypsin was digested at 37°C for 30 minutes, gently shaken for 5 times during this period, sieved, and centrifuged to collect cell suspension and inoculated in T75cm 2 In the culture flask, cultured in cell culture medium A for 5 to 7 days, and the spindle-like cells grew adherently; 3) After trypsinization, the adherent growing cells were further cultured, passaged, purified, amplified and passaged for 6 passages and harvested. After concentrating and preparing a cell suspension with cell preservation solution, some cells are sent for quality inspection, and the rest are stored in a liq...
Embodiment 2
[0078] Isolation of CD151 by Adherent Subculture + CD184 - Oct4 + subpluripotent stem cells
[0079] The same as in Example 1, except that the cell culture medium used was cell culture medium B.
[0080] The obtained results were substantially the same as in Example 1.
Embodiment 3
[0082] Isolation of CD151 by Adherent Subculture + CD184 - Oct4 + subpluripotent stem cells
[0083] The same as in Example 1, except that the cell culture medium used was cell culture medium C.
[0084] The obtained results were substantially the same as in Example 1.
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