Sub-totipotent stem cell, preparation method and application thereof

A technology of sub-totipotent stem cells and uses, applied in the field of sub-totipotent stem cells, the preparation of sub-totipotent stem cells, can solve the problems of poor understanding of stem cells

Active Publication Date: 2012-10-03
BEIJING HEALTH & BIOTECH (H&B) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, people's understanding and clinical use of multipotent stem cells such as hematopoietic stem cells is relatively deep, and the understanding of embryonic stem cells has also made great progress. Many characteristics of stem cells, but also have many biological characteristics of pluripotent stem cells, transplanted into the body will not produce teratomas, such a type of stem cells are poorly understood

Method used

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  • Sub-totipotent stem cell, preparation method and application thereof
  • Sub-totipotent stem cell, preparation method and application thereof
  • Sub-totipotent stem cell, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Isolation of CD151 by Adherent Subculture + CD184 - Oct4 + subpluripotent stem cells

[0074] Cut off human placenta for CD151 + CD184 - Oct4 + Preparation of stem cell products. The specific steps are as follows: 1) Aseptically collect the cut human placenta; 2) Cut to small fragments 3 Small tissue pieces were digested with 0.5 mg / mL collagenase I at 37°C for 1 hour, and 1 mg / mL trypsin was digested at 37°C for 30 minutes, gently shaken for 5 times during this period, sieved, and centrifuged to collect cell suspension and inoculated in T75cm 2 In the culture flask, cultured in cell culture medium A for 5 to 7 days, and the spindle-like cells grew adherently; 3) After trypsinization, the adherent growing cells were further cultured, passaged, purified, amplified and passaged for 6 passages and harvested. After concentrating and preparing a cell suspension with cell preservation solution, some cells are sent for quality inspection, and the rest are stored in a liq...

Embodiment 2

[0078] Isolation of CD151 by Adherent Subculture + CD184 - Oct4 + subpluripotent stem cells

[0079] The same as in Example 1, except that the cell culture medium used was cell culture medium B.

[0080] The obtained results were substantially the same as in Example 1.

Embodiment 3

[0082] Isolation of CD151 by Adherent Subculture + CD184 - Oct4 + subpluripotent stem cells

[0083] The same as in Example 1, except that the cell culture medium used was cell culture medium C.

[0084] The obtained results were substantially the same as in Example 1.

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Abstract

The invention discloses a sub-totipotent stem cell from cut human umbilical cord or placenta, wherein the cell marker is CD151<+>OCT4<+>CD184<->; the cell grows adhering to the wall in a culture container and can differentiate to the body entoderm, mesoblast and ectoderm tissues. The invention also discloses a preparation method of the sub-totipotent stem cell and an application thereof for preparing drug for treating cell injuries or cell aging diseases, and an application thereof as a carrier cell of a gene treatment drug.

Description

technical field [0001] The present invention relates to the field of stem cells, in particular to a subpluripotent stem cell derived from cut human umbilical cord or placenta. At the same time, the present invention also relates to the preparation method of the subpluripotent stem cell and the use of the subpluripotent stem cell. Background technique [0002] The human body is made up of trillions of cells. Cells are the basic structural and functional units of the human body and are differentiated from stem cells. Therefore, stem cells are the origin cells of various tissues and organs of the human body. Stem cells are characterized by self-replication and multidirectional differentiation. During normal reproductive physiology, stem cells differentiate from fertilized eggs. The fertilized egg initially develops into primordial embryonic stem cells, which have the full potential to form a complete individual. Primitive embryonic stem cells are further differentiated and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735A61K48/00A61P39/06A61P7/06A61P39/00A61P37/02A61P25/00A61P37/06
Inventor 赵宝娜
Owner BEIJING HEALTH & BIOTECH (H&B) CO LTD
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