Method for induction of test-tube seedling Dioscorea Opposita Thunb. beans and seedling formation

A technology of yam beans and test-tube seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low reproduction coefficient, low survival rate of tissue culture seedling propagation and transplanting, etc., and achieve high reproduction coefficient, transplanting Save time and effort, high survival rate effect

Inactive Publication Date: 2012-10-24
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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Problems solved by technology

[0004] The purpose of the present invention is to find a method for increasing the multiplication coefficient of yam seed potatoes, which is relatively simple, convenient, stable and time-saving. This method has the advantages of high multiplication coefficient, high transplanting survival rate, time saving, The advantages of not being affected by environmental conditions can make up for the shortcomings of the existing low reproductive coefficient of yam tubers and the low survival rate of tissue culture seedling propagation and transplanting, thus providing a new method for the propagation of yam seed tubers

Method used

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  • Method for induction of test-tube seedling Dioscorea Opposita Thunb. beans and seedling formation
  • Method for induction of test-tube seedling Dioscorea Opposita Thunb. beans and seedling formation
  • Method for induction of test-tube seedling Dioscorea Opposita Thunb. beans and seedling formation

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Embodiment Construction

[0017] The present invention will be further illustrated below through the examples of inducing the formation of yam beans based on test-tube seedlings and then propagating seed potatoes.

[0018] The experimental material of this embodiment is the tissue-cultured seedling of Tieguan Huaiyam.

[0019] 1. Yam tissue culture seedlings induce the formation of yam beans

[0020] The yam stems with axillary buds were sterilized and inoculated with 0.5-1 mg·l -1 6-BA, 0-0.1 mg·l -1 Adventitious buds were induced in the MS medium of NAA, and when the germinated adventitious buds grew to two or more leaves, a single bud was cut and inoculated in a medium containing 1.5 mg·l -1 6-BA, 0mg·l -1 Subcultured in MS medium of NAA, after multiplication, the robust seedlings with a height of more than 2.0 cm and 2 leaves were excised, and transferred to a culture medium containing 1.0 mg·l -1 6-BA, 0.2mg·l -1 NAA, mass ratio of 0.02% activated carbon MS medium for rooting culture, and...

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Abstract

Belonging to the field of biotechnologies, the invention discloses a method for induction of test-tube seedling Dioscorea Opposita Thunb. beans and seedling cultivation. According to the method, first, Dioscorea Opposita Thunb. test-tube seedling induction is conducted to form Dioscorea Opposita Thunb. beans, which are then directly cultivated into seedlings without breaking dormancy, and then factory production for breeding of Dioscorea Opposita Thunb. seed yams can be realized. A medium for inducing Dioscorea Opposita Thunb. test-tube seedlings to form Dioscorea Opposita Thunb. beans consists of 1L of 1/2MS culture medium, 0.2mg of 6-BA, 1.0mg of NAA, 10-20g of cane sugar, 7g of agar, and 0.02% of activated carbon. After cultivation of the medium for 20-30d, the Dioscorea Opposita Thunb. beans can form. When the test-tube seedlings are induced to form Dioscorea Opposita Thunb. beans, the Dioscorea Opposita Thunb. beans are inoculated into a seedling formation medium made up of MS, 1.5mg.l<-1> of 6-BA, and 0.2mg.l<-1> of NAA. After about 20d, budding can be induced. And after further cultivation for 1 month, seedlings can be formed.

Description

technical field [0001] The present invention "method for induction and seedling growth of yam beans in test-tube seedlings" is to induce yam beans from test-tube seedlings of yams, and then propagate seed potatoes through yam beans, which belongs to the field of biotechnology. Background technique [0002] Chinese yam (Dioscorea Opposita Thunb.) is a perennial herbaceous winding vine of the genus Dioscorea in the family Dioscorea, and it is planted in all parts of the north and south of my country. The subterranean fleshy rhizomes and the leaf axils of the above-ground branches (yam beans) are all products, which are used for people's food or medicine. Because of its high nutritional value and medicinal value, it is an authentic medicinal material and traditional export commodity in my country, and its products sell well in Southeast Asia, Japan and other countries. However, due to the long-term use of vegetative propagation, its quality is degraded and its yield is reduced...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 殷剑美韩晓勇张培通彭琦郭文琦
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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