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Screening method of fenpropathrin pesticide degradation bacterium

A technology for degrading fenothrin and pesticides, which is applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of high cost, long time consumption, large workload, etc., and achieves low cost, small workload, The effect of heavy workload

Inactive Publication Date: 2012-10-31
GUIYANG UNIV
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Problems solved by technology

However, the current screening methods for degrading bacteria are mainly to obtain fenpropathrin pesticide-degrading bacteria through a series of means such as collecting samples, enriching culture, separating and purifying, gradient domestication culture, and determining the degradation rate, which takes a long time, a large workload, and costs high

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  • Screening method of fenpropathrin pesticide degradation bacterium
  • Screening method of fenpropathrin pesticide degradation bacterium

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Embodiment 1

[0020] A screening method for fenpropathrin pesticide-degrading bacteria adopts the following steps: (1) collecting samples; (2) enriching culture; (3) selecting medium for culturing; (4) separating and purifying; (5) obtaining degrading strains ,preservation.

[0021] The specific steps of the screening method are as follows: take the activated sludge collected near the wastewater outlet of the pesticide factory and place it in 100 mL of basal salt medium. The formula of the basal salt medium: fenpropathrin: 100 mg·L -1 , NaCl: 1.0 g / L, NH 4 NO 3 : 1.0 g / L, K 2 HPO 4 : 1.5 g / L, KH 2 PO 4 : 0.5 g / L, MgSO 4 ·7H 2 O: 0.1 g / L, Ph=7.0, at 37 °C, 130 r min -1 Cultivate on a shaker for 1 day, transfer to the same medium with an inoculum of 5 mL, transfer 3 times in total, take 1 mL of the enrichment solution and add it to the selection medium, the selection medium culture formula: fenpropathrin: 600 mg·L -1 , NaCl: 1.0 g / L, NH 4 NO 3 : 1.0 g / L, K 2 HPO 4 : 1.5 g / L, KH ...

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Abstract

The invention discloses a screening method of a fenpropathrin pesticide degradation bacterium, which comprises the following steps: acquiring a sample; carrying out enrichment culture; selecting a culture medium, and culturing; separating and purifying; and obtaining the degradation bacterial strain, and preserving. The method overcomes the defects of time consuming, high workload and high cost in the traditional fenpropathrin pesticide degradation bacterium, is simple and convenient, shortens the screening time, and reduces the workload for screening.

Description

technical field [0001] The invention relates to a screening method for fenpropathrin pesticide-degrading bacteria. Background technique [0002] The accumulation of the pesticide fenpropathrin in large quantities in the soil will affect the biological chains of crops, livestock and humans, and will lead to the decline of soil quality and yield and affect the corresponding economic income. The bioremediation technology based on microorganisms can be used to degrade various organic pollutants, and has the advantages of high efficiency, safety, no secondary pollution, and low cost. The fenpropathrin pesticide degrading bacteria can be used to degrade and remove the pesticide fenpropathrin residues , can achieve the purpose of reducing the detoxification and increasing the yield, and play the role of ensuring food safety and increasing social benefits. However, the current screening method for degrading bacteria is mainly to obtain fenpropathrin pesticide-degrading bacteria th...

Claims

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Application Information

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IPC IPC(8): C12N1/00
Inventor 余磊武思齐赵杰
Owner GUIYANG UNIV
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