Gene group test structure

Abstract

A gene group test structure at least comprises a gene test chip, a specimen pretreatment unit, a hybridization area, a chip color development unit and an analyzing and identifying unit. In this way, a WEnCA-Chipball operating platform, which is high in speed, accuracy and sensitivity and low in cost, allows quantitative analysis and is easy to combine with automation, is provided to conduct rapid test and analysis of a biological specimen, achieving the aim which cannot be realized by conventional biomedical test.

Description

technical field

[0001] The present invention relates to a gene group detection structure, especially relates to an improvement of the shortcomings of the original nylon diaphragm operating platform, especially refers to the establishment of a new low-cost WEnCA (Weighted Enzymatic Chip Array) operating platform, which can be assisted by automatic operation to enable detection The time is greatly reduced, and human error is reduced, so as to break through the bottleneck faced by chip detection technology in the commercialization process. Background technique

[0002] Analysis of gene overexpression has led to fundamental and clinical advances in disease diagnosis. The techniques for studying gene transition expression include Northern Blot, Reverse Transcription Polymerase Chain Reaction (RT-PCR) and real-time quantitative polymerase chain reaction (Real-Time PCR). ). Among them, Northern Blot is limited to research operations and cannot be practically applied to clinical ...

Examples

Embodiment Construction

[0031] see figure 1 and figure 2 Shown are a schematic diagram of the gene group detection structure of the present invention and a schematic diagram of gene position arrangement of the gene detection chip of the present invention, respectively. As shown in the figure: the present invention is a gene group detection structure (WEnCA-Chipball) 100, which at least includes a gene detection chip 10, a sample pre-processing unit 20, a hybrid reaction area 30, a chip color rendering unit 40 and an analysis and interpretation unit 50 constitute.

[0032] The above-mentioned gene detection chip 10 is coated with a specific sequence, and is a screening chip with complete disease diagnosis, drug efficacy evaluation or genetic disease-causing genes.

[0033] The sample pretreatment unit 20 adds the sample to the lysis buffer to break the cells, extracts the mRNA, and binds the RNA in the cell by adding magnetic particles (Magnetic Particles) containing Poly-T primers, and lyses the n...

technical field

[0001] The present invention relates to a gene group detection structure, especially relates to an improvement of the shortcomings of the original nylon diaphragm operating platform, especially refers to the establishment of a new low-cost WEnCA (Weighted Enzymatic Chip Array) operating platform, which can be assisted by automatic operation to enable detection The time is greatly reduced, and human error is reduced, so as to break through the bottleneck faced by chip detection technology in the commercialization process. Background technique

[0002] Analysis of gene overexpression has led to fundamental and clinical advances in disease diagnosis. The techniques for studying gene transition expression include Northern Blot, Reverse Transcription Polymerase Chain Reaction (RT-PCR) and real-time quantitative polymerase chain reaction (Real-Time PCR). ). Among them, Northern Blot is limited to research operations and cannot be practically applied to clinical ...

Examples

Embodiment Construction

[0031] see figure 1 and figure 2 Shown are a schematic diagram of the gene group detection structure of the present invention and a schematic diagram of gene position arrangement of the gene detection chip of the present invention, respectively. As shown in the figure: the present invention is a gene group detection structure (WEnCA-Chipball) 100, which at least includes a gene detection chip 10, a sample pre-processing unit 20, a hybrid reaction area 30, a chip color rendering unit 40 and an analysis and interpretation unit 50 constitute.

[0032] The above-mentioned gene detection chip 10 is coated with a specific sequence, and is a screening chip with complete disease diagnosis, drug efficacy evaluation or genetic disease-causing genes.

[0033] The sample pretreatment unit 20 adds the sample to the lysis buffer to break the cells, extracts the mRNA, and binds the RNA in the cell by adding magnetic particles (Magnetic Particles) containing Poly-T primers, and lyses the n...

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