Preparation method of high-concentration acrylamide aqueous solution by microbiology

The technology of acrylamide and microorganism method is applied in the field of preparation of high-concentration microorganism method acrylamide aqueous solution, which can solve the problems of large environmental pollution, easy self-polymerization, high cost, etc., so as to improve the utilization rate, meet the concentration requirements, and improve the market competition. force effect

Inactive Publication Date: 2012-11-14
江苏南天农科化工有限公司
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Problems solved by technology

[0002] There are chemical and biological methods for the production of acrylamide. The chemical method generally produces 16% to 20% of the content, and the microbial hydration method generally has a concentration of 25 to 30%. With the development of the acrylamide industry, the market is demanding high-concentration acrylamide The demand for acrylamide is increasing. Generally, the demand for acrylamide with a concentration of 40-50% is relatively large. Some special customers require a maximum concentration of 55-60%. Generally, when customers need high-concentration acrylamide, they basically use acrylamide dry powder to dissolve water It can be prepared by dissolving or heating and concentrating with 30% acrylamide, which is costly and pollutes the environment, and the concentration process is easy to self-polymerize

Method used

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Embodiment Construction

[0013] Concrete implementation steps of the present invention are as follows:

[0014] 1. Bacterial strain cycle screening: Firstly, induce and domesticate the Nocardia strain to obtain an excellent strain, and improve the tolerance of the strain through fed-batch culture and repeated tests of anti-substrate product inhibition , to obtain a strain with a tolerance increase of 30% and an enzyme activity as high as 25 million U / ml.

[0015] 2. Preparation of free cells: For the strains screened in step 1, under sterile conditions, inoculate the strains into the fermentation medium for cultivation, the temperature is 28±2°C, the stirring speed is set at 150~200r / min, and the aeration The volume is 40L / min, and fermented for 40-50h to obtain the fermented liquid, and the fermented liquid is centrifuged with a high-speed disc centrifuge to remove water-soluble substances and impurities in the fermented liquid, and the separated solid bacteria are dehydrated through a microfiltratio...

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Abstract

The invention discloses a preparation method of high-concentration acrylamide aqueous solution by microbiology. The preparation method includes strain domestication and screening, free cell preparation, and multistage continuous serial reaction. The preparation method has the advantages that the common one-step intermittent reaction is replaced by the multistage continuous serial reaction, acrylamide is produced from the free cells by microbiological hydration, processes of reactors are controlled, bacteria are separated, utilization of the strain is increased, 60% high-concentration acrylamide can be obtained, the requirements of market for acrylamide are met, and market competitiveness of products is improved.

Description

technical field [0001] The invention relates to a preparation method of high-concentration microbial acrylamide aqueous solution. Background technique [0002] There are chemical and biological methods for the production of acrylamide. The chemical method generally produces 16% to 20% of the content, and the microbial hydration method generally has a concentration of 25 to 30%. With the development of the acrylamide industry, the market is demanding high-concentration acrylamide The demand for acrylamide is increasing. Generally, the demand for acrylamide with a concentration of 40-50% is relatively large. Some special customers require a maximum concentration of 55-60%. Generally, when customers need high-concentration acrylamide, they basically use acrylamide dry powder to dissolve water It can be prepared by dissolving or heating and concentrating with 30% acrylamide, which is costly and pollutes the environment, and the concentration process is easy to self-polymerize. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02C12R1/365
Inventor 熊光辉沈瑞华杨涛陆煌生顾稀平韩浩梁丁建华水瑞芬
Owner 江苏南天农科化工有限公司
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