Bacillus SSAL-6 and application thereof in degrading anabaena flos-aquae

A technology of blooming Anabaena and Bacillus, applied in the field of microbiology, can solve rare and endangering human problems

Inactive Publication Date: 2013-11-27
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Anabaena anabaena (a common algae that causes water blooms) is one of the main algal species that cause eutrophication in water bodies. It is widely distributed and can produce algal toxins, which directly and indirectly harm humans. The study of Anabaena sinensis is very rare

Method used

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  • Bacillus SSAL-6 and application thereof in degrading anabaena flos-aquae
  • Bacillus SSAL-6 and application thereof in degrading anabaena flos-aquae
  • Bacillus SSAL-6 and application thereof in degrading anabaena flos-aquae

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Embodiment 1

[0014] Example 1, Isolation, Purification and Identification of Bacillus SSAL-6

[0015] 1. Isolation and purification of Bacillus SSAL-6

[0016] Using the yellowed Anabaena anabaena liquid from the Key Laboratory of Urban Agriculture (South) of the Ministry of Agriculture as the source of isolation of algae-dissolving bacteria, the smearing plate method and the partitioning method were used to purify and separate the 25 strains of bacteria obtained Place them in 100mL LB liquid medium respectively, the shaker speed is 180r min -1 , cultured at 37°C for 24 hours, and then 800 μL was added dropwise to the solid plate of Anabaena algae blooms, and the algae-dissolving effect of each bacteria was compared through the inspection of the size of the algae-dissolving spot, and finally the strain with a higher algae-dissolving effect was screened out SSAL-6. It was identified as Bacillus and named Bacillus SSAL-6. Put it into the slant medium and store it in the refrigerator at 4°...

Embodiment 2

[0023] Example 2, Bacillus Bacillus SSAL-6 on the growth effect of Anabaena blooms and the influence of photosynthetic pigments

[0024] 1. Effect of Bacillus SSAL-6 on the growth effect of Anabaena blooms

[0025] Anabaena blooms were cultivated with reference to the standard method of algae growth inhibition experiment (Ministry of Environmental Protection), using Shuisheng No. 111 nitrogen-free culture medium, with a pH of 7.5. The culture temperature is 30±2°C, the light is continuously illuminated for 24 hours, the light intensity is 3000±200lx, the culture is static, and it is shaken regularly 3 times a day.

[0026] Anabaena concentration in blooms is 5 x 10 4 ~1×10 5 Bacillus SSAL-6 was added to 100mL of the algae solution (thalline concentration was about 10 9 per mL), the treatment concentration (v / v) gradient was: 5%, 10%, 15%, 20%, 25%, 30% and 35%, and each group of samples was set with 3 replicates. Prepare the same series of different proportions of culture ...

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Abstract

The invention discloses a Bacillus SSAL-6 and an application thereof in degrading anabaena flos-aquae. The bacillus is obtained by separating and screening yellowed anabaena flos-aquae in Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, is named as Bacillus SSAL-6, and is deposited in China General Microbiological Culture Collection Center with the accession number of CGMCC No.6195 on 6 June 2012. Under natural conditions, the degradation effect of Bacillus SSAL-6 on the anabaena flos-aquae increases with the increase of the strain concentration, when the concentration of the bacillus is 35%, the inhibition rate of anabaena flos-aquae chlorophyll alpha can reach 96%. The Bacillus SSAL-6 disclosed herein has good effect of degrading algae, can effectively degrade the anabaena flos-aquae, provides an effective means for controlling water body eutrophication, and provides an important method for utilizing microbes to treat water bloom.

Description

technical field [0001] The invention relates to a microorganism, in particular to a strain of bacillus Bacillus SSAL-6 and its application in degrading anabaena blooms, belonging to the technical field of microorganisms. Background technique [0002] Eutrophication of water bodies has become a common environmental problem plaguing countries all over the world. The massive reproduction of planktonic algae leads to frequent outbreaks of algae blooms, seriously affecting human life, production and health, and causing ecological disasters worldwide. It is imminent to explore effective ways to control the occurrence of algae blooms. At present, physical and chemical methods are mainly used to control water eutrophication, but these two methods will not only consume a lot of financial and material resources, but also destroy the ecological environment to a certain extent. As the control organisms of algae blooms and red tides, alginolytic bacteria have been widely concerned by en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07
Inventor 沈健英孙秀敏
Owner SHANGHAI JIAOTONG UNIV
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