Paraffin texture chip preparation method

A technology of tissue chip and production method, which is applied in the preparation of test samples, etc., can solve the problems of low slice rate, low operation efficiency, complicated tissue chip process, etc., and achieve high slice rate, widely popularized and used, and clear cells visible effect

Inactive Publication Date: 2012-11-28
王虎
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  • Abstract
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Problems solved by technology

[0008] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a method for producing a ...

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  • Paraffin texture chip preparation method
  • Paraffin texture chip preparation method
  • Paraffin texture chip preparation method

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Embodiment Construction

[0031] In order to make the object, technical solution and effect of the present invention more clear and definite, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0032] In view of the above existing problems, the present invention provides a paraffin tissue chip manufacturing method, which is a fast, simple, economical and applicable manufacturing method, especially for tissue microchips with a diameter of less than 0.5mm, which is accurate and reliable. The method for making a tissue chip is a method for making a paraffin tissue microchip that is cross-isolated and glued and fixed. Specifically, it is positioned by punching holes, and the method of arranging and isolating needles and threads with glue is used, and then embedding in paraffin, cooling, and using The release agent removes the needles and fus...

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Abstract

The invention discloses a paraffin texture chip preparation method which is a rapid, simple, economic and applicable preparation method, particularly aiming at the preparation of a texture microchip of which the diameter is less than 0.5mm; and the preparation method is precise and reliable. The preparation method specifically comprises the steps of punching holes and positioning, using a needle wire attached gel arranging isolating method, carrying out paraffin embedding, cooling, taking down the needle by using a release agent, and performing refusion so as to obtain the texture microchip. The paraffin texture chip prepared by using the preparation method has the advantages of being tidy in arrangement, uniform in interval, and high in chip preparation rate after being sliced, economic, practical, and low in cost, and cells are clear and visible without damage after being dyed, so that the texture microchip technology can be popularized and used more widely.

Description

technical field [0001] The invention relates to the field of tissue chip preparation, in particular to a method for making a paraffin tissue chip. Background technique [0002] Tissue microchip technology is a miniature biochemical analysis system that integrates the discontinuous analysis process of life science on the surface of the glass chip according to its biological characteristics, so as to realize the analysis of cells, proteins, genes and other organisms. Accurate, rapid, and large-information detection and screening of components. [0003] Biological microchips are a revolutionary tool for new drug discovery. In view of the rules of new drug R&D and marketing, once a new drug is finally approved by the FDA, it must be screened from the initial 10,000 compounds. Only 1% of the compounds may enter animal experiments, and only 5-3 of them may enter human trials. In the end, only one was approved for marketing. [0004] The research and development costs before an ...

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Application Information

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IPC IPC(8): G01N1/36
Inventor 王虎
Owner 王虎
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