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Biomass hydrolysis process

A process, hydrolase technology, applied in the field of process, can solve problems such as profitability limitation

Active Publication Date: 2012-11-28
NOVOZYMES AS +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of enzymes used in hydrolysis has been seen as a limitation to the profitability of such processes

Method used

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  • Biomass hydrolysis process
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Corn stover was pretreated using steam explosion at 205°C for 5 minutes. The resulting PCS (pretreated corn stover) was washed with tap water to remove soluble inhibitors. Fed-batch hydrolysis was performed with washed PCS at 15%, 20% and 30% TS (total solids), respectively. All batches of washed PCS started with 12.6% TS and required 1, 3 or 4 additional loads of PCS to achieve the specified final TS of 15%, 20% or 30%. The cellulase preparation was added at 0 hours at an enzyme dose of 6 mg EP / g cellulose, or split in half and half added at 0 hours and the other half at 24 hours. Hydrolysis was carried out at 50°C and pH 5.0 for 96 hours.

[0090] Samples of the total slurry were drawn every 24 hours and the sugar concentration was determined by HPLC. The results are shown in Table 1. Glucose equivalent conversion was calculated as the percentage of cellobiose and glucose produced out of the total glucose potential in the PCS.

[0091]

Embodiment 2

[0093] The above tests were repeated to obtain data for about 25% TS content, except that the reactor setup was slightly different. In Example 1 a vertical reactor setup was used, while in this example a horizontal reactor was used. Generally, this will result in lower sugar yields.

[0094]

[0095]

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Abstract

The invention relates to a biomass process comprising split dosage of the cellulose hydrolysing enzyme.

Description

technical field [0001] The present invention relates to a process comprising the use of split dosing of cellulolytic enzymes in the hydrolysis of lignocellulose-containing material. Background of the invention [0002] Due to limited reserves of fossil fuels and concerns about greenhouse gas emissions, there is increasing concern about the use of renewable energy sources, such as fermentation products, such as bioethanol. Ethanol production from biomass, ie, lignocellulose-containing material, is known in the art and may include pretreatment, enzymatic hydrolysis and fermentation of lignocellulose-containing material to ethanol. The cost of enzymes used in hydrolysis has been seen as a limitation to the profitability of such processes. Therefore, there is a need to provide improved and more efficient processes for enzymatic hydrolysis of lignocellulose-containing materials into substrates suitable for fermentation. [0003] The inventors of the present application have now...

Claims

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Application Information

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IPC IPC(8): C12P7/06C12P7/10
CPCY02E50/17Y02E50/16Y02E50/10
Inventor W.韩李冬敏M.刘
Owner NOVOZYMES AS