Vector type scanning method of laser scanning confocal microscope

A technology of confocal microscope and scanning method, applied in the field of vector scanning of laser scanning confocal microscope, can solve the problems of reducing the service life of the galvanometer system, violent vibration, accelerated aging of the galvanometer coating, etc., to improve the overall imaging speed, The effect of prolonging the service life and reducing the adjustment time of the galvanometer

Active Publication Date: 2012-12-12
SUZHOU INST OF BIOMEDICAL ENG & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in order to effectively reduce the adjustment time of the galvanometer during the scanning process, a fast and large-scale return is required. However, the scanning galvanometer vibrates violently during this process, which accelerates the aging of the galvanometer coating and reduces the service life of the galvanometer system.

Method used

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  • Vector type scanning method of laser scanning confocal microscope
  • Vector type scanning method of laser scanning confocal microscope
  • Vector type scanning method of laser scanning confocal microscope

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] refer to Figure 4 Shown, vector scanning method of the present invention comprises:

[0047] S11, performing a quick pre-scan on the sample;

[0048] S21. Perform ROI identification on the sample after the quick pre-scan;

[0049] S31. Generate a vector scan path for the sample based on the ROI identification, and the generated scan path covers the sample ROI;

[0050] S41. Carry out scanning path judgment on the vector scanning path; the scanning path judgment is to judge whether the range of the vector scanning path meets the scanning requirements, and if so, execute step S61;

[0051] S51. If not, adjust the sample ROI, and execute step S31;

[0052] S61. Carry out fine vector scanning of the ROI of the sample according to the vector scanning path: under the drive of the controller, rotate the scanning vibrating mirror according to the vector scanning path to obtain a scanned image of the sample.

[0053] The scanning requirements are: the scanning path covers all...

Embodiment 2

[0055] refer to Figure 5 Shown, vector scanning method of the present invention comprises:

[0056] S1, carry out fast pre-scanning (shown in figure 5b) to sample (shown in figure 5a);

[0057] S2. Perform ROI identification on the sample after the quick pre-scan (shown in 5c in the figure);

[0058] S3. Generate an outer spiral scan path for the sample based on the ROI identification (shown in 5d in the figure), and the generated scan path covers the sample ROI;

[0059] S4. Carry out vector fine scanning of the sample ROI according to the outer helical scanning path (shown in 5d in the figure): driven by the controller, the scanning vibrating mirror is rotated according to the vector scanning path to obtain a scanned image of the sample.

[0060] The above scanning process may also include steps S41: Scanning path judgment, S51: Sample ROI adjustment, S61: Vector fine scanning according to Embodiment 1, that is, the vector scanning path in Embodiment 1 is an outer spiral ...

Embodiment 3

[0063] refer to Image 6 Shown, vector scanning method of the present invention comprises:

[0064] S11, performing a quick pre-scan (shown in 6b in the figure) to the sample (shown in 6a in the figure);

[0065] S21. Perform ROI identification on the sample after the quick pre-scan (shown in 6c in the figure);

[0066] S31. Generate an inner helical scanning path for the sample based on the ROI identification (shown in 6d in the figure), and the generated scanning path covers the sample ROI;

[0067] S41. Carry out scanning path judgment on the inner helical scanning path; in the scanning path judgment, judge whether the range of the outer helical scanning path meets the scanning requirements, and if yes, execute step S61;

[0068] S51. If not, adjust the sample ROI, and execute step S31;

[0069] S61. Carry out vector fine scanning of the sample ROI according to the inner helical scanning path (shown in 6d in the figure): driven by the controller, the scanning galvanomete...

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Abstract

The invention relates to a vector type scanning method of a laser scanning confocal microscope. The method comprises the following steps: pre-scanning a sample rapidly; carrying out ROI (region of interest) identification on the pre-scanned sample; carrying out vector type scanning path generation on the sample according to the ROI identification, wherein the generated scanning path covers the sample ROI; carrying out vector fine scanning on the sample ROI according to the vector type scanning path; and under a driving function of a controller, rotating a scanning galvanometer according to the vector type scanning path and obtaining a scanning image of the sample by the laser scanning confocal microscope.

Description

【Technical field】 [0001] The invention relates to a scanning imaging method of a laser scanning confocal microscope, in particular to a vector scanning method of a laser scanning confocal microscope. 【Background technique】 [0002] Laser scanning confocal microscope (Laser Scanning Confocal Microscope, LSCM) uses the laser point as the excitation light of fluorescence and continuously scans the specimen through the scanning device, and blocks the out-of-focus plane light through the space conjugate aperture (pinhole) to form an image. A kind of microscope; laser scanning confocal microscope is an effective technical means for studying submicron microstructure, and it is a necessary large-scale scientific research instrument for scientific and technological workers engaged in biomedical and material science research. [0003] Such as figure 1 Shown is the double-galvanometer X-Y scanning mechanism of the laser scanning confocal microscope, including a controller and an X-Y s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 黄维张运海薛晓君高飞张龙陈瑞涛
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH
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