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Application of miRNA (micro ribonucleic acid) 320a in prostate cancer serological diagnostic kit
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A rnamir-320a, prostate cancer technology, applied in the field of biomedical materials
Inactive Publication Date: 2012-12-19
NANKAI UNIV
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Although many candidate biomarkers are being discovered continuously, no new indicators and methods have been applied to clinical detection
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Embodiment 1
[0031] The present invention firstly provides an application of a reagent for detecting microRNA miR-320a and its precursor in a blood sample in the preparation of a kit for diagnosing and selecting a treatment plan for prostate cancer. 320a (as shown in SEQ ID NO: 1) and reagents specific to its precursors, specifically: PCR primers and probes specific to miR-320a and its precursors and genes prepared using the probes chip.
[0032] Described specific primer is selected from the sequence shown in SEQ ID NO:2;
[0033] The specific probe is selected from the sequence shown in SEQ ID NO:5.
Embodiment 2
[0034] Embodiment 2, prostate cancer diagnostic kit
[0035] The target nucleic acid detected by the kit of the present invention is miR-320a having a polynucleotide sequence shown in SEQ ID NO: 1, specifically comprising:
[0043] 1. Using the xenograft model invented by us (i.e. transplanting prostate cancer tissue into the kidneycapsule of immunodeficient mice,
[0044] The obtained tissue lines are highly correlated with the pathological features of clinical tumors) and next-generation sequencing technology, for metastatic and non-metastatic
[0045] miRNAs in metastatic prostate cancer xenograft tissue lines (surgical specimens from the same prostate cancer patient)
[0046] Transcriptomics has been extensively studied. By comparison we identified 104 differentially expressed miRNAs, except
[0047] Some known miRNAs and their isoforms (isomiRs), and some new ones that have not yet been discovered
[0048] miRNAs. Among them, 21 miRNAs have been found to be related to the occurrence of prostate cancer, and 5 miRNAs have been
[0049] It has been reported to play a role in the metastasis of prostate cancer, which also proves the effectiv...
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Abstract
The invention relates to an application of an miRNA (micro ribonucleic acid, miR-320a, shown in SEQIDNO: 1) in the prostatecancer serological diagnostic kit, in particular to an application of reagents detecting miR-320a of a blood sample in the preparation of the object prostatecancer diagnostic kit, wherein the reagents are reagents specific to the miRNA (miR-320a) and the precursor of the miRNA; and particularly, the reagents are a specific probe detecting the miR-320a and the precursor, and a polymerasechain reaction (PCR) primer. The invention also relates to a prostatecancer diagnostic kit which contains a PCR reactive reagent, a miR-320a specific primer, a nematode miR-39 reference oligonucleotide sequence, a nematode miR-39 specific primer, a fluorescent dye SYBR-GREEN1 and an operating manual. When the detection result shows that the level of the miR-320a or precursor in the object blood is higher than that in the control serum, the object is shown to suffer from prostate cancer, thus the kit can be effectively used in the auxiliary diagnosis of clinical prostate cancer.
Description
technical field [0001] The invention relates to the application of a microRNA (micro RA, miRNA, miR) 320a in a prostate cancer serological diagnosis kit. It belongs to the technical field of biomedical materials. Background technique [0002] Prostate cancer (PCa) is one of the common malignant tumors and leading causes of death in men in European and American countries. [0003] With the extension of life expectancy and the westernization of lifestyle in my country, the incidence of PCa is increasing year by year, and it is listed as one of the fastest growing tumors in China in the 21st century. Based on the 19 up-regulated miRNAs sequences screened in the previous work, this project will further detect their content in clinical serum samples to find serological biomarkers in patients with human prostatecancer metastasis. [0004] At present, the research work on the diagnostic indicators of prostate cancer mainly focuses on the following aspects: (I) PSA subtypes and o...
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