Method for purifying vitamin k-dependent proteins such as coagulation factor ix

A vitamin-dependent technology, applied in coagulation/fibrinolytic factors, peptide preparation methods, chemical instruments and methods, etc., can solve expensive problems, achieve the effect of inhibiting aggregates and maintaining natural molecular structure
CN102858971BInactive Publication Date: 2016-06-01OCTAPHARMA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
OCTAPHARMA
Publication Date
2016-06-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method for the preparation of prion-free vitamin K-dependent proteins in a purification sequence using chromatography, characterized in that: - at least one chromatographic step is performed using a multimodal resin; - vitamin K-containing dependent proteins are provided as an aqueous solution fraction of protein; - contacting fraction containing vitamin K-dependent protein with multimodal resin at pH 6-9; - optional washing with aqueous wash buffer prior to elution of vitamin K-dependent protein Multimodal resin for vitamin K-dependent proteins to wash away contaminants and retain vitamin K-dependent proteins; -Elute vitamin K-dependent from multimodal resins at pH 6-9 in arginine-containing buffers protein; and - optionally collecting the vitamin K-dependent protein-containing fraction in purified or enriched form.
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Description

technical field

[0001] The present invention relates to a method for purifying a vitamin K-dependent protein such as blood coagulation factor IX (abbreviated as FIX) and a fraction comprising a vitamin K-dependent protein such as FIX obtainable by the method of the present invention. Background technique

[0002] Hemophilia is a genetic disorder that impairs the body's ability to control blood clotting, or blood clotting. In one of its forms, hemophilia B, a deficiency of blood clotting factor IX, or FIX for short, occurs in about 1 in 25,000 males. Factor IX (or Christmas factor) is a serine protease of the coagulation system. It is a vitamin K-dependent plasma protein, through the Ca 2+ Participates in coagulation by converting factor X to its active form in the presence of ions, phospholipids and factor VIIIa. FIX proteins are essential factors of blood coagulation with multifunctional properties.

[0003] Factor IX (FIX) is a single-chain glycoprotein comprising 461 ...

Claims

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