Preparation method of corn active peptides

An active peptide, corn technology, applied in the biological field, can solve the problems of restricting development and high cost, and achieve the effects of low price, improved digestibility and improved protein conversion rate

Inactive Publication Date: 2013-01-09
SHAANXI TONGZHENG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The synthesis method is just in the research stage in China, and active peptides are prepared by liquid phase or s...

Method used

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  • Preparation method of corn active peptides

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Experimental program
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Effect test

Embodiment 1

[0022] (1) Pretreatment: Put 100g of corn gluten powder into a miniature plant sample pulverizer with a crushing chamber diameter of 105mm and a rotating speed of 1000r / min for pulverization, and sieve with an 80-mesh sieve. In the oscillating reactor, use anhydrous sodium carbonate with a mass concentration of 50g / L, add 20mL / g, treat for 15min, then centrifuge at 6000rpm / min for 15min, repeat washing with water for 3 times, centrifuge at 6000rpm / min for 15min and then hydrolyze;

[0023] (2) Extrusion and puffing: the zein is modified by using a split twin-screw extruder, the die hole diameter is 15mm, the sleeve temperature is 85°C, the moisture content of the material is 10%, and the screw speed is 120r / min;

[0024] (3) Ultrasonic enzymolysis: take puffed corn gluten powder with a mass fraction of 10%, break the wall for 15 minutes under 420W ultrasonic conditions, stir at 50-55°C, adjust the pH value to 9-10, add 3% enzyme, and use it under this condition Alcalase alkali...

Embodiment 2

[0030] (1) Pretreatment: Put 100g of corn gluten powder into a miniature plant sample pulverizer with a crushing chamber diameter of 105mm and a rotating speed of 1000r / min for pulverization, and sieve with an 80-mesh sieve. In the shaking reactor, use anhydrous sodium carbonate with a mass concentration of 60g / L, add 10mL / g, treat for 15min, then centrifuge at 6000rpm / min for 15min, wash with water repeatedly for 3 times, centrifuge at 6000rpm / min for 15min and then hydrolyze;

[0031] (2) Extrusion and puffing: the zein is modified by using a split twin-screw extruder with a die hole diameter of 15mm, a sleeve temperature of 85°C, a moisture content of the material of 12%, and a screw speed of 120r / min;

[0032] (3) Ultrasonic enzymolysis: take puffed corn gluten powder with a mass fraction of 12%, break the wall for 12 minutes under 430W ultrasonic conditions, stir at 50-55°C, adjust the pH value to 9-10, add 4% enzyme, and use it under this condition Alcalase alkaline endo...

Embodiment 3

[0038] (1) Pretreatment: Put 100g of corn gluten powder into a miniature plant sample pulverizer with a crushing chamber diameter of 105mm and a rotating speed of 1500r / min for pulverization, and sieve with an 80-mesh sieve. In the oscillating reactor, use anhydrous sodium carbonate with a mass concentration of 60g / L, add 15mL / g, treat for 15min, then centrifuge at 7000rpm / min for 13min, repeat washing with water for 3 times, centrifuge at 7000rpm / min for 13min and then hydrolyze;

[0039] (2) Extrusion and puffing: the zein is modified by using a split twin-screw extruder, the die hole diameter is 15mm, the sleeve temperature is 85°C, the moisture content of the material is 15%, and the screw speed is 130r / min;

[0040] (3) Ultrasonic enzymolysis: take puffed corn gluten powder with a mass fraction of 13%, break the wall for 13 minutes under 440W ultrasonic conditions, stir at 50-55°C, adjust the pH value to 9-10, add 4% enzyme, and use it under this condition Alcalase alkali...

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Abstract

The invention discloses a preparation method of corn active peptides. The preparation method includes subjecting corn protein powder to steps of pretreating, extrusion puffing, ultrasonic enzymolysis, enzyme deactivation, debittering, decoloring, gel chromatography, freeze-drying and the like. The preparation method is simple in process and low in cost. Content (by dry basis) of prepared corn active peptides reaches more than 85%, yield reaches more than 72%, and a feasible process for large-scale production is provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of corn active peptide. Background technique [0002] Corn active peptide is a hydrolyzed product of corn protein, which is composed of short peptides with small molecular weight but high activity, and generally a mixed oligopeptide with a molecular weight below 1000. Although oligopeptides have a small number of polymerized amino acids, they can produce the role of higher-order structures. Corn peptide is superior to amino acid or protein absorption, and is completely soluble in water under a wide range of pH values, without turbidity and sedimentation. At the same time, it is extremely stable to heat and does not lose its function. It has the functions of lowering blood pressure, protecting the liver, resisting high cholesterol, promoting fat metabolism, delaying aging, and enhancing immune function. It is safe and reliable, and has no toxic and side effects. T...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
Inventor 武昌厚
Owner SHAANXI TONGZHENG TECH CO LTD
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