Method for extracting tea saponin from tea-seed pancake
A technology of camellia seed cake and tea saponin, which is applied in the field of deep processing of camellia seed products, can solve the problems of many extractants, low product purity, low tea saponin purity, etc., and achieves fast extraction speed, high product purity, and high extraction rate. Effect
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Embodiment 1
[0019] Step 01: After crushing the tea seed cake and drying it in the sun, weigh 75 grams of tea seed cake powder, put it into a 500 ml Soxhlet extractor, add 200 ml of isopropanol extractant containing 0.1% water, and heat Reflux extraction for 1.5 hours;
[0020] Step 02: Distill all the extracts separated from the Soxhlet extractor in Step 01, recover 145ml of isopropanol as the extractant, and the residue after distillation is crude tea saponin;
[0021] Step 03: Transfer the crude tea saponin obtained in step 02 into an evaporating dish, heat it on a water bath to constant weight, and obtain 9.5 grams of refined tea saponin, the purity of which is greater than 97.3%;
[0022] Step 04: Heat the residue remaining in the Soxhlet extractor in step 01 with a water bath, steam the extractant isopropanol remaining in the residue, recover 45ml of isopropanol, and the recovered isopropanol in step 02 and step 04 can be used in step 04 01 Recycle.
Embodiment 2
[0024] Step 01: After crushing the tea seed cake and drying it in the sun, weigh 75 grams of tea seed cake powder, put it into a 500 ml Soxhlet extractor, add 75 ml of isopropanol extractant containing 0.2% water, and heat Reflux extraction for 2 hours;
[0025] Step 02: Distill all the extracts separated from the Soxhlet extractor in Step 01, recover 55ml of isopropanol as the extractant, and the residue after distillation is crude tea saponin;
[0026] Step 03: Transfer the crude tea saponin obtained in step 02 into an evaporating dish, heat it on a water bath to constant weight, and obtain 4.1 grams of refined tea saponin, the purity of which is greater than 85%;
[0027] Step 04: Heat the residue remaining in the Soxhlet extractor in step 01 with a water bath, steam the extractant isopropanol remaining in the residue, recover 10ml of isopropanol, and the recovered isopropanol in step 02 and step 04 can be used in step 04 01 Recycle.
Embodiment 3
[0029] Step 01: After crushing the tea seed cake and drying it in the sun, weigh 75 grams of tea seed cake powder, put it into a 500 ml Soxhlet extractor, add 175 ml of isopropanol extractant containing 0.2% water, and heat Reflux extraction for 2 hours;
[0030] Step 02: Distill all the extracts separated from the Soxhlet extractor in Step 01, recover 140ml of isopropanol as the extractant, and the residue after distillation is crude tea saponin;
[0031] Step 03: Transfer the crude tea saponin obtained in step 02 into an evaporating dish, heat it on a water bath to constant weight, and obtain 8.8 grams of refined tea saponin, the purity of which is greater than 95%;
[0032] Step 04: Heating the residue remaining in the Soxhlet extractor in step 01 with a water bath, steaming out the extractant isopropanol remaining in the residue, recovering 16ml of isopropanol, and the recovered isopropanol in step 02 and step 04 can be used in step 04 01 Recycle.
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