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Method for synthesizing nano silver by utilizing Bacillus amyloliquefaciensBacillus amyloliquefaciens LSSE-62

A technology of amylolytic spores and nano-silver, which is applied in the field of microorganisms, can solve the problems of restricting industrial applications, not conforming to green chemistry, and slow synthesis speed, and achieves the effects of high stability, fast synthesis speed, and high-efficiency antibacterial activity

Inactive Publication Date: 2013-01-23
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the bacterial synthesis speeds reported so far are relatively slow, and it takes 24-120 hours to reduce 1mM silver nitrate, which greatly limits its industrial application.
Studies have found that intestinal bacteria (such as Klebsiella pneumoniae) can rapidly synthesize nano-silver under t

Method used

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  • Method for synthesizing nano silver by utilizing Bacillus amyloliquefaciensBacillus amyloliquefaciens LSSE-62
  • Method for synthesizing nano silver by utilizing Bacillus amyloliquefaciensBacillus amyloliquefaciens LSSE-62
  • Method for synthesizing nano silver by utilizing Bacillus amyloliquefaciensBacillus amyloliquefaciens LSSE-62

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 synthesizes nano-silver particle by bacterial extract

[0046] a. Fermentation culture

[0047] Inoculate Bacillus amyloliquefaciens LSSE-62 into LB liquid seed medium (containing peptone 10g / L, yeast powder 5g / L, NaCl 10g / L, pH 7.2), culture at 37°C, 180rpm for 12h, according to 3% (v / w) inoculum amount, added to LB liquid medium, cultivated at 37°C, 200rpm for 24h; centrifuged at 10000rpm to collect the bacteria, washed twice, ultrasonically broken, and centrifuged to remove cell debris, and the cell extract was used for nano-silver synthesis matrix.

[0048] b. Nano silver synthesis

[0049] Add 1 mM silver nitrate solution to the cell extracts at concentrations of 1, 2, 3, and 4 mg / mL, and react for 100 min under sunlight irradiation (70000 lv).

[0050] c. Characterization of nano silver

[0051] The synthesis of silver nanoparticles can be characterized by color change and its strong absorption in the visible region, figure 1 It shows that the r...

Embodiment 2

[0052] Embodiment 2 synthesizes nano-silver particle by bacterial extract

[0053] a. Fermentation culture

[0054] Inoculate Bacillus amyloliquefaciens LSSE-62 into LB liquid seed medium (containing peptone 10g / L, yeast powder 5g / L, NaCl 10g / L, pH 7.2), culture at 37°C, 180rpm for 12h, according to 3% (v / w) inoculum amount, added to LB liquid medium, cultivated at 37°C, 200rpm for 24h; centrifuged at 10000rpm to collect the bacteria, washed twice, ultrasonically broken, and centrifuged to remove cell debris, and the cell extract was used for nano-silver synthesis matrix.

[0055] b. Nano silver synthesis

[0056] Add 1mM silver nitrate solution to the cell extract with a concentration of 3mg / mL, add 0.5, 1, 2, 3mM NaCl, and react for 100min under sunlight irradiation (70000lv).

[0057] c. Characterization of nano silver

[0058] The synthesis of silver nanoparticles can be characterized by color change and its strong absorption in the visible region, attached image 3 ...

Embodiment 3

[0059] Embodiment 3 synthesizes nano-silver particle by bacterial extract

[0060] a. Fermentation culture

[0061] Inoculate Bacillus amyloliquefaciens LSSE-62 into LB liquid seed medium (containing peptone 10g / L, yeast powder 5g / L, NaCl 10g / L, pH 7.2), culture at 37°C, 180rpm for 12h, according to 3% (v / w) inoculum amount, added to LB liquid medium, cultivated at 37°C, 200rpm for 24h; centrifuged at 10000rpm to collect the bacteria, washed twice, ultrasonically broken, and centrifuged to remove cell debris, and the cell extract was used for nano-silver synthesis matrix.

[0062] b. Nano silver synthesis

[0063] Add 1 mM silver nitrate solution to the cell extract with a concentration of 4 mg / mL, and react for 100 min under sunlight irradiation (30000, 40000, 50000, 70000 lv).

[0064] c. Characterization of nano silver

[0065] The synthesis of silver nanoparticles can be characterized by color change and its strong absorption in the visible region, attached Figure 5...

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Abstract

The invention relates to the field of microorganism and in particular relates to a method for synthesizing nano silver by utilizing Bacillus amyloliquefaciens LSSE-62. The method comprises the following steps of: 1) fermenting and cultivating the Bacillus amyloliquefaciens LSSE-62, and separating to obtain thallus and fermented supernate; and 2) taking the thallus and fermented supernate obtained in the step 1) as reaction matrix, adding AgNO3 solution to cause concentration of the reaction matrix in the obtained mixed solution to be 1-4mg/mL and concentration of AgNO3 to be 1-3mM, and reacting under the condition of sunniness or ultraviolet irradiation, so as to obtain nano silver particles, wherein the preservation number of the Bacillus amyloliquefaciens LSSE-62 is CGMCC No.4157. The synthetic method disclosed by the invention is safe and has no toxic or harm effect, synthesis speed is high, and reaction can be completed within 80-100min, so that the synthetic method is efficient, safe and simple.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular, the invention relates to a method for synthesizing nano-silver by using bacillus amyloliquefaciens LSSE-62. Background technique [0002] In recent years, metal nanoparticles have been increasingly used in various fields, such as electronics, medicine and biotechnology. As a unique metal nanoparticle, nanosilver has attracted the attention of researchers in the fields of antibacterial materials, therapeutics, biomolecular detection and catalysis. [0003] Green chemistry requires the development of safe, environmentally friendly and energy-saving green technologies. Traditional chemical methods have been widely used in the synthesis of nano-silver, but the toxic substances and high temperature conditions involved in these methods do not meet the principles of green chemistry (see reference 1 for details: P.Quaresma, L.Soares, L.Contar, A . Miranda, I. Osorio, P.A. Carvalho, R. Franc...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/07
Inventor 刘会洲魏雪团罗明芳谢渝春刘德明
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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