Non-contact gene amplification electrochemical quick detection method

Abstract

The present invention relates to the field of bioengineering, and relates specifically to a contactless gene amplification electrochemical rapid detection method, applicable in gene amplification process monitoring and result analysis. A change in the ionic strength of an amplification reaction solution is measured by coupling a contactless electrical conductivity sensor via a capacitor, combined with a reaction time factor, and qualitative and quantitative information of target gene fragment in a sample DNA or RNA is produced in a simple, rapid, and automated manner at reduced costs.

Description

Technical field: [0001] The invention relates to the field of bioengineering, in particular to a non-contact gene amplification electrochemical rapid detection method. Background technique: [0002] For gene amplification, whether the reaction product is specific and whether the result is accurate and reliable depends on strict analysis and identification techniques. At present, the detection / monitoring and analysis techniques of amplification products and amplification processes can be divided into the following categories: [0003] 1. Electrophoresis [see literature Ihira M, Yoshikawa T, Enomoto Y, Akimoto S, Ohashi M, Suga S, Nishimura N, Ozaki T, Nishiyama Y, Notomi T, Ohta Y, Asano Y: Rapid Diagnosis of Human Herpesvirus 6 Infection by a Novel DNA Amplification Method, Loop-Mediated Isothermal Amplification, J. Clin. Microbiol. 2004, 42(1), 140-145]. Including agarose gel electrophoresis, polyacrylamide gel electrophoresis and related enzyme cutting-gel electrophoresi...

AI Technical Summary

Problems solved by technology

Blot hybridization and dot hybridization based on visual hybridization results, although the method is simple and the equipment is cheap, they also have the same disadvantages as the electrophoresis method: the use of dyes, long cycle, risk of process contamination, poor quantitative effect, only endpoint analysis, etc.

The optical method effectively avoids the possibility of the product polluting the environment, making it possible to monitor the progress of the amplification reaction in real time and perform accurate quantitative analysis, but there are still disadvantages that cannot be ignored: there are strict requirements on the transparency of the relevant reagent solutions and containers; optical equipment is expensive ;Optical / electrical conversion device is required to output the results conveniently and automatically; the use of probes will significantly increase the operating cost

[0009] Although various current methods can complete the result analysis or process monitoring of the amplification reaction in a certain direction and within a certain range, they are not ideal in terms of low cost, high throughput, automation and miniaturization.

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