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Biological grease in algae cell and preparation method and application of biological grease

A technology of algae cells and bio-oil, which is applied in the field of biodiesel preparation, can solve the problems that the production scale and output are difficult to meet the huge demand, and achieve the effects of easy automatic control, wide source of raw materials, and easy scale

Inactive Publication Date: 2014-01-22
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the United States, the main raw material for biodiesel is soybean oil, but its production scale and output have been difficult to meet the huge demand

Method used

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  • Biological grease in algae cell and preparation method and application of biological grease

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A kind of biological oil in algae cell is prepared by the method comprising the following steps:

[0042] (1) Measure 200 ml of the harvested algae liquid into a 1 L autoclave, and stir for 5 min at 120 r / min;

[0043] (2) Turn on the ozone generator and air compressor, control the total flow rate of ozone and air to 2 L / min according to the volume ratio, that is, ozone: air ratio of 1:150, and pass the mixed gas composed of ozone and air into the In the high-pressure reactor, continue to stir the algae liquid at a constant speed of 120 r / min, so that the incoming ozone, air and algae liquid are evenly mixed;

[0044] (3) Control the pressure of the high-pressure reactor to 0.6 MPa. When it reaches 0.6 MPa, open the exhaust valve of the high-pressure reactor to reduce the pressure to normal pressure, and then increase the pressure to 0.6 MPa. When it reaches 0.6 MPa, open it again The exhaust valve of the high-pressure reactor reduces the pressure to normal pressure, a...

Embodiment 2

[0052] A kind of biological oil in algae cell is prepared by the method comprising the following steps:

[0053] (1) Measure 200 mL of harvested algae liquid into a 1 L autoclave, and stir for 5 min at 120 r / min;

[0054] (2) Turn on the ozone generator and air compressor, control the total flow rate of ozone and air to 2 L / min according to the volume ratio, that is, ozone: air ratio of 1:150, and pass the mixed gas composed of ozone and air into the In the high-pressure reactor, continue to stir the algae liquid at a constant speed of 120 r / min, so that the incoming ozone, air and algae liquid are evenly mixed;

[0055] (3) Control the pressure of the high-pressure reactor to 0.6 MPa. When it reaches 0.6 MPa, open the exhaust valve of the high-pressure reactor to reduce the pressure to normal pressure, and then increase the pressure to 0.6 MPa. When it reaches 0.6 MPa, open it again The exhaust valve of the high-pressure reactor reduces the pressure to normal pressure, and t...

Embodiment 3

[0063] A kind of biological oil in algae cell is prepared by the method comprising the following steps:

[0064] (1) Measure 200 mL of harvested algae liquid into a 1 L autoclave, and stir for 5 min at 120 r / min;

[0065] (2) Turn on the ozone generator and air compressor, control the total flow rate of ozone and air to 2 L / min according to the volume ratio, that is, ozone: air ratio of 1:150, and pass the mixed gas composed of ozone and air into the In the high-pressure reactor, continue to stir the algae liquid at a constant speed of 120 r / min, so that the incoming ozone, air and algae liquid are evenly mixed;

[0066] (3) Control the pressure of the high-pressure reactor to 0.8 MPa. When it reaches 0.8 MPa, open the exhaust valve of the high-pressure reactor to reduce the pressure to normal pressure, and then increase the pressure to 0.8 MPa. When it reaches 0.8 MPa, open it again The exhaust valve of the high-pressure reactor reduces the pressure to normal pressure, and t...

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Abstract

The invention discloses a method for preparing biological grease in algae cell. The method comprises the following steps of: firstly, crushing the algae cell, collecting mixed liquor of crushed algae cell, performing centrifugation, taking supernatant, performing first-time liquid-liquid extraction by using a normal hexane / ethyl acetate mixed organic solvent, and collecting aqueous phase and organic phase; secondly, performing second-time liquid-liquid extraction on the aqueous phase obtained by the first-time liquid-liquid extraction by using the normal hexane / ethyl acetate mixed organic solvent, and collecting aqueous phase and organic phase; thirdly, performing third-time liquid-liquid extraction on the aqueous phase obtained by the second-time liquid-liquid extraction by using the normal hexane / ethyl acetate mixed organic solvent, and collecting aqueous phase and organic phase; fourthly, combining the organic phases obtained by the three-time extraction, and performing vacuum evaporation to obtain the biological grease in algae cell; and finally adding a KOH-containing methanol solution into the biological grease in algae cell, stirring violently, and performing esterification reaction to obtain biodiesel which is in line with 'GB / T 20828-2007 Bildiesel Blend Stock (BD100) for Diesel Engine Fuels'.

Description

technical field [0001] The invention relates to a bio-oil in algae cells and its application in preparing bio-diesel. Background technique [0002] With the accelerated consumption of fossil fuels and petroleum energy worldwide and various pollution and environmental problems caused by it, renewable bio-energy has been paid more and more attention by people. With the encouragement and support of various policies, the world's annual production of biofuels (including biodiesel and bioethanol, etc.) has reached about 35 billion liters. [0003] Biodiesel is a long-chain fatty acid methyl ester obtained by transesterification of bio-oil (usually including vegetable fat, animal fat, etc.) and alcohols (usually methanol, ethanol) as raw materials. At a certain temperature, methanol transesterifies with triglycerides in bio-oils to produce glycerol and fatty acid methyl esters. After glycerol is separated, the obtained fatty acid methyl esters are biodiesel. The theoretical molar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/10C11B1/04C11B3/12C11C3/04C10L1/02
CPCY02E50/13Y02E50/10
Inventor 李亮黄远星许智华金小颖李洋洋方学友宋翠红崔晨晨
Owner UNIV OF SHANGHAI FOR SCI & TECH
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