IgY antibody preparation having mixed specificity for resistance to streptococcus mutans and streptococcus sobrinus, and preparation method and IgY total toothpaste

A technology of Streptococcus distalis and Streptococcus mutans, applied in the fields of antibody, antibacterial immunoglobulin, dentistry, etc.

Active Publication Date: 2013-03-20
上海美加净日化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Technicians cannot know in advance that anti-Porphyromonas gingivalis and Fusobacterium nucleatum mixed IgY are more e

Method used

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  • IgY antibody preparation having mixed specificity for resistance to streptococcus mutans and streptococcus sobrinus, and preparation method and IgY total toothpaste
  • IgY antibody preparation having mixed specificity for resistance to streptococcus mutans and streptococcus sobrinus, and preparation method and IgY total toothpaste
  • IgY antibody preparation having mixed specificity for resistance to streptococcus mutans and streptococcus sobrinus, and preparation method and IgY total toothpaste

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Experimental program
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Embodiment 1

[0039] Preparation of mixed specific IgY antibody preparations against Streptococcus mutans and Streptococcus distalis:

[0040] A. Streptococcus mutans and Streptococcus farus culture steps

[0041] A.1 Bacterial species Bacterial species: Streptococcus mutans (S.mutans) and Streptococcus farus (S.sobrinus);

[0042] A.2 Streptococcus mutans (S.mutans) MT8148 strain and S. sobrinus (S. sobrinus) 6715 strain were taken, and the Streptococcus mutans and Streptococcus mutans and Streptococcus sobrinus were isolated and amplified by BHI-S medium respectively, And it was confirmed as Streptococcus mutans and Streptococcus mutans to obtain Streptococcus mutans thallus and thalline fragments, Streptococcus aureus thallus and thallus fragments;

[0043] B. Immunogen preparation steps:

[0044] B.1 Take 10-70 parts by weight of Streptococcus mutans cells and cell fragments, and 30-90 parts by weight of Streptococcus mutans cells and cell fragments and mix them into bacterial liqui...

Embodiment 2

[0055] Preparation of mixed specific IgY antibody preparations against Streptococcus mutans and Streptococcus distalis:

[0056] A. Streptococcus mutans and Streptococcus farus culture steps

[0057] A.1 Bacterial species Bacterial species: Streptococcus mutans (S.mutans) and Streptococcus farus (S.sobrinus);

[0058] A.2 Take Streptococcus mutans (S.mutans) MT8148 strain and Streptococcus mutans (S.sobrinus) 6715 strain, which were isolated and cultured and amplified by BHI-S medium respectively. And it was confirmed as Streptococcus mutans and Streptococcus mutans to obtain Streptococcus mutans thallus and thalline fragments, Streptococcus aureus thallus and thallus fragments;

[0059] B. Immunogen preparation steps:

[0060] B.1 Take 10-70 parts by weight of Streptococcus mutans cells and cell fragments, and 30-90 parts by weight of Streptococcus mutans cells and cell fragments and mix them into bacterial liquid antigen;

[0061] B.2 Mix an equal volume of bacterial fl...

Embodiment 3

[0071] Preparation of mixed specific IgY antibody preparation against Porphyromonas gingivalis and Fusobacterium nucleatum:

[0072] A. Porphyromonas gingivalis and Fusobacterium nucleatum culture steps

[0073] A.1 Bacterial species: Porphyromonas gingivalis and Fusobacterium nucleatum;

[0074] A.2 Porphyromonas gingivalis (P.g) ATCC33277 strain and Fusobacterium nucleatum (Fn) ATCC10953 strain were taken, and the Porphyromonas gingivalis and Fusobacterium nucleatum (Fn) ATCC10953 strains were separated and cultured on BHI-S medium, respectively. bacilli, and were identified and confirmed as Porphyromonas gingivalis and Fusobacterium nucleatum to obtain Porphyromonas gingivalis cells and cell fragments, Fusobacterium nucleatum cells and cell fragments;

[0075] B. Immunogen preparation steps:

[0076]B.1 Take 10-70 parts by weight of Porphyromonas gingivalis cells and cell fragments, and 30-90 parts by weight of Fusobacterium nucleatum cells and cell fragments and mix th...

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Abstract

The invention discloses an IgY antibody preparation having mixed specificity for resistance to streptococcus mutans and streptococcus sobrinus. The IgY antibody preparation is prepared by mixing 10-70 parts by weight of streptococcus mutans bacteria and bacteria pieces and 30-90 parts by weight of streptococcus sobrinus bacteria and bacteria pieces into an antigen via laying hens. The invention also discloses a preparation method of the IgY antibody preparation having the mixed specificity for resistance to the streptococcus mutans and the streptococcus sobrinus, and a toothpaste which contains the IgY antibody preparation having the mixed specificity for resistance to the streptococcus mutans and the streptococcus sobrinus and the IgY antibody preparation having the mixed specificity for resistance to the anti-porphyromonas gingivalis and the fusobacterium nucleatum and is used for preventing saprodontia, gingivitis and halitosis thereof. The toothpaste used for preventing saprodontia, gingivitis, periodontitis and halitosis halitosis thereof has obvious effects for preventing saprodontia, gingivitis, periodontitis and halitosis bacteria thereof.

Description

technical field [0001] The present invention relates to the technical field of oral products, in particular to a preparation containing mixed specific IgY antibodies against Streptococcus mutans and Streptococcus distantis and a preparation method thereof, and a preparation containing mixed specific IgY antibodies against Streptococcus mutans and Streptococcus distantis, Toothpaste for prevention of dental caries and gingivitis and bad breath with mixed specific IgY antibody preparation of Fusobacterium nucleatum and Porphyromonas gingivalis. Background technique [0002] In my country, the dental caries rate of children aged 3-6 years is 25.49%-92.72%, and that of adults is 40.37%. Among them, Streptococcus mutans and Streptococcus distalis are the main cariogenic bacteria. The occurrence of bacterial infectious diseases firstly settles in specific organs in the body through the specific binding of adhesins bound to the surface of bacteria to their corresponding receptors ...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61K8/99C07K16/12A61P1/02A61Q11/00
Inventor 陈健芬罗鑫龙孙晓青张萍
Owner 上海美加净日化有限公司
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