Duck tembusu virus (DTMUV) E protein gene and application thereof

A duck Tembusu virus, protein technology, applied in the direction of application, virus peptide, antiviral agent, etc.

Active Publication Date: 2013-03-20
QINGDAO BOITE BIOPHARM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Currently, there are no effective meas...

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  • Duck tembusu virus (DTMUV) E protein gene and application thereof
  • Duck tembusu virus (DTMUV) E protein gene and application thereof
  • Duck tembusu virus (DTMUV) E protein gene and application thereof

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Embodiment Construction

[0009] The present invention isolates the new Duck Tembusu virus E gene from DTMUV disease materials, and the recombinant protein obtained through gene expression has good antigenicity and can defend against the new Duck Tembusu virus, thereby contributing to the present invention.

[0010] The present invention will be further described below in conjunction with specific embodiments, but those skilled in the art should understand that the details and forms of the technical solution of the present invention can be modified or replaced without departing from the technical solution of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0011] 1. Amplification of Duck Tembusu virus E gene

[0012] The clinically isolated DTMUV disease material was treated and inoculated with SPF chicken embryos for three generations of blind passage, and the full-length gene of Duck Tambusu virus E was amplified by PCR. ...

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Abstract

The invention relates to a duck tembusu virus (DTMUV) E protein gene, and the amino acid sequence of the DTMUV E protein gene is SEQ ID NO:1. The DTMUV genetic engineering subunit vaccine obtained by the invention is inoculated to DTMUV negative Sheldrake in the age of 20 days by virtue of muscles, blood is sampled and a DTMUV antibody is determined 14 days after inoculation, DTMUV antibodies in an immunization group are all positive, the effective rate reaches up to 100%, and no antibody is detected in a control group. Results show that the prepared recombinant protein has good immunogenicity, and further the prepared DTMUV genetic engineering subunit vaccine can arouse immune response of the Sheldrake.

Description

technical field [0001] The invention belongs to the technical field of poultry genetic engineering vaccines, and in particular relates to a duck Tembusu virus E protein gene and an application thereof. Background technique [0002] Duck Tembusu virus (DTMUV) is an emerging virus that can cause duck egg production decline, growth retardation and death. The mortality rate of the disease is between 5% and 10%. It is one of the most serious diseases that have endangered the duck industry since 2010. According to the survey in November 2010 of the National Waterfowl Industry Technology System, the economic loss caused by the disease has exceeded 5 billion. At present, there are no effective measures to prevent the occurrence of the disease. Therefore, the development of a safe and effective "duck Tembusu virus disease" vaccine is of great significance to the development of duck industry in my country. [0003] Studies have found that duck Tembusu virus encodes structural prote...

Claims

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Application Information

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IPC IPC(8): C07K14/01C12N15/34A61K39/12A61P31/20
Inventor 魏波凌红丽徐丽丽蒋贻海于春梅
Owner QINGDAO BOITE BIOPHARM
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