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High-pressure cell incubator, temperature control method and pressure control method thereof

A cell culture and temperature control device technology, applied in the field of high-pressure cell incubators, can solve problems such as explosions and achieve the effect of making up for temperature changes

Inactive Publication Date: 2013-03-20
NAVY MEDICINE RES INST OF PLA
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Problems solved by technology

For example: most of the existing cell incubators are directly heated by resistance wires, which may cause safety hazards such as explosions under the high-voltage environment of the resistance wires

Method used

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  • High-pressure cell incubator, temperature control method and pressure control method thereof
  • High-pressure cell incubator, temperature control method and pressure control method thereof
  • High-pressure cell incubator, temperature control method and pressure control method thereof

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Embodiment Construction

[0055] As described in the background technology, the way to provide a temperature environment for cell growth under normal pressure is to use resistance wires to directly heat, and this method is prone to explosion in a high-pressure environment. In order to avoid the above situation, the present invention uses a water jacket to cover the box The surface inside the body uses the water circulation in the water jacket to flow, so that the temperature of the box body can be maintained by providing heat to the water flow in the water jacket.

[0056] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and understandable, the specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. Since the emphasis is on explaining the principle of the present invention, the drawings are not drawn to scale.

[0057] 1) First introduce the structure of the high-pressure ce...

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Abstract

The invention relates to a high-pressure cell incubator. The high-pressure cell incubator comprises a box body, and a water jacket for providing a working temperature for the box body, wherein the water jacket covers on the inner surface of the box body. The invention also provides a temperature control method and a pressure control method of the high-pressure cell incubator. By adoption of the technical scheme of the high-pressure cell incubator, a growing environment for cells under high pressure can be provided.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a high-pressure cell culture box, a temperature control method and a pressure control method of the high-pressure cell culture box. Background technique [0002] Isolating the required cells and amplified cells from multicellular organisms, as well as in vitro transformation and observation of cells, must first solve the problem of cell culture in vitro. Compared with microbial cell culture, it is more difficult to obtain single cells from multicellular organisms. Cell culture, especially the culture of animal (including human) cells. The function of the cell incubator is to provide an environment for cell growth. Specifically, the general conditions for cell growth include: 1) temperature, 2) pH value, 3) nutrients, and 4) pressure. For the first three conditions, there are some related technologies in the existing technology. Taking human cells as an example, the suitable temperat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/36C12Q3/00
Inventor 方以群陈杰包晓辰王芳芳姚健陈海庭张师陈亮
Owner NAVY MEDICINE RES INST OF PLA
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