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Application method of cyclic peptide compounds extracted from cortex lycii radicis

A technology of cyclic peptide compounds and application methods, which is applied in the application field of preparing hypoglycemic or treating diabetes preparations

Inactive Publication Date: 2013-03-27
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of peptides or cyclic peptides with insulin-like effects for hypoglycemic therapy is less

Method used

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  • Application method of cyclic peptide compounds extracted from cortex lycii radicis
  • Application method of cyclic peptide compounds extracted from cortex lycii radicis
  • Application method of cyclic peptide compounds extracted from cortex lycii radicis

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Remove the dust and impurities from Digupi, wash, dry, and crush to about 30 mesh, weigh 3000g of Digupi powder, add 2.5 times the amount (volume to mass ratio mL / g) 30-60°C boiling range Petroleum ether, heated in a water bath at 55-65°C and continuously refluxed (reflux time 4-5 hours), extracted 2-3 times, combined the extracts, recovered the solvent, and evaporated the solvent to obtain the Digustifolia petroleum ether extract; Put the bone skin residue in a ventilated place to remove petroleum ether, then add 3 times the amount (volume to mass ratio, mL / g) of methylene chloride, heat in a water bath at 65-75°C and continuously reflux (reflux time 4-5 hours) to extract 2-3 Once, combine the extracts, reclaim the solvent, and evaporate the solvent to obtain the dichloromethane extract of Digustifolia; the dichloromethane residue after the dichloromethane extraction is placed in a ventilated place to remove the dichloromethane, and then add 2 to 3 times the amount of m...

Embodiment 2

[0050] A, B, C and D cyclic peptide compound hypoglycemic effect test obtained in Example 1:

[0051] First, 8-week-old Sprague-Dawley rats (initial body weight 200-250g) were fed high-fat diet for 3 months, and then intraperitoneally injected small doses of streptozocin for 1 week to induce blood glucose in normal rats to rise On this basis, the oral glucose tolerance test (OGTT) was used to inject cyclic peptides A, B, C and D drug samples 5 mg / Kg (body weight) into the tail vein respectively, and pioglitazone, a drug recognized in Western medicine for treating diabetes, was positive. As a control, the effects of A, B, C and D cyclic peptide compounds on the body weight, blood sugar and insulin of diabetic rats were observed.

[0052] Then, 12 hours after the third day of stopping the intraperitoneal injection of streptozotocin, the fasting blood glucose was measured, and the fasting blood glucose>11.1mmol / L was taken as the successful criterion of modeling. The 48 rats suc...

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Abstract

The invention relates to an application method of cyclic peptide compounds extracted from cortex lycii radicis. The method comprises the following steps of: continuously carrying out silicagel column chromatography on the cortex lycii radicis carbinol extractive, carrying out the polystyrene microporous resin crude separation and the inverse phase C18 silica gel column chromatography refining, and carrying out freeze drying to obtain four cyclic peptide compounds. The abdominally-dosed rat oral glucose tolerance test shows that the cyclic peptide compounds have the function of an insulin sample, and are higher in a blood sugar reducing effect. When the dose is 5mg / kg (weight), the inhibition ratio of the cyclic peptide compounds to the rise of the postprandial 2h blood sugar concentration of the diabetic rats induced by the streptozotocin can reach 76.5%, 67.4%, 65.8% and 69.3% respectively, therefore, the cyclic peptide compounds can be used for preparing the drug for treating diabetes or reducing blood sugar.

Description

technical field [0001] The invention relates to an application method of a cyclic peptide compound extracted from Cortex Digustifolia, in particular to the application of the cyclic peptide compound as a preparation for hypoglycemia or treatment of diabetes, which can be used to develop a therapeutic or auxiliary treatment for diabetes. medicine. Background technique [0002] Cortex Lycii radicis is the root bark of Lycium chinense Mill. and Lycium barbarum L., a plant of the Solanaceae family. It is generally used for clearing deficiency-heat, purging lung-fire, and cooling blood. Indications for yin deficiency and labor-heat, bone steaming and night sweats, malnutrition and fever in children, lung heat and coughing, vomiting blood, epistaxis, hematuria, and quenching thirst. At the same time, modern pharmacological studies have shown that Digupi has a wide range of physiological activities, such as lowering blood sugar, lowering blood pressure, lowering blood fat, antiba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/12A61P3/10
Inventor 谢练武郭亚平孙汉洲胡云楚
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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