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A method for extracting hypoglycemic components from seabuckthorn leaves

A seabuckthorn leaf and hypoglycemic technology, which is applied to medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulas, etc., can solve problems such as difficult separation, complex ingredients, and low content, and achieve strong reduction Excellent sugar effect, hypoglycemic effect

Active Publication Date: 2018-02-13
SHANXI JINKEHAI BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2014, the pentacyclic triterpenoids in seabuckthorn were determined for the first time. Due to the complex composition of this kind of substances, the components of some analogues are not easy to separate, so the content is based on the amount of corosolic acid, which is 0.05% in seabuckthorn , the content is low, so far there is no report on its enrichment
The hypoglycemic effect of this level of extract is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting hypoglycemic components from seabuckthorn leaves, according to the following steps:

[0020] 1. Pre-processing

[0021] After the seabuckthorn leaves are cleaned and dried, they are crushed into a coarse powder, and water 15 times the weight of the coarse powder is added to boil, cooled to room temperature, and the sea buckthorn leaf pre-boiled liquid is obtained;

[0022] 2. Insulation soaking

[0023] Add 3wt% Daqu according to the mass of the pre-cooking liquid, mix well, and keep warm for 3 days at 26-30°C;

[0024] 3. Drying

[0025] Filter the pre-boiled liquid after heat preservation and soaking, and dry the filter residue to obtain seabuckthorn leaf powder, which is set aside;

[0026] 4. Extraction

[0027] According to the mass ratio of ethanol extract: seabuckthorn leaf powder = 5:1, use the extract to reflux extract the seabuckthorn leaf powder at a temperature of 80-90°C and reflux twice; after the reflux extraction, recover the ...

Embodiment 2

[0030] A method for extracting hypoglycemic components from seabuckthorn leaves, according to the following steps:

[0031] 1. Pre-processing

[0032] After the seabuckthorn leaves are cleaned and dried, they are crushed into a coarse powder, and water 10 times the weight of the coarse powder is added to boil, and cooled to room temperature to obtain the seabuckthorn leaf pre-boiled liquid;

[0033] 2. Insulation soaking

[0034] Add 2wt% Daqu according to the mass of the pre-cooking liquid, mix well, and keep warm at 30-35°C for 5 days;

[0035] 3. Drying

[0036] Filter the pre-boiled liquid after heat preservation and soaking, and dry the filter residue to obtain seabuckthorn leaf powder, which is set aside;

[0037] 4. Extraction

[0038] According to the mass ratio of ethanol extract: seabuckthorn leaf powder = 5:1, use the extract to reflux extract the seabuckthorn leaf powder at a temperature of 80-90°C and reflux 3 times; after the reflux extraction, recover the ex...

Embodiment 3

[0041] A method for extracting hypoglycemic components from seabuckthorn leaves, according to the following steps:

[0042] 1. Pre-processing

[0043] After the seabuckthorn leaves are cleaned and dried, they are crushed into a coarse powder, and water 20 times the weight of the coarse powder is added to boil, and cooled to room temperature to obtain the seabuckthorn leaf pre-boiled liquid;

[0044] 2. Insulation soaking

[0045] Add 5wt% Daqu according to the mass of the pre-cooking liquid, mix well, and keep warm at 32-36°C for 5 days;

[0046] 3. Drying

[0047] Filter the pre-boiled liquid after heat preservation and soaking, and dry the filter residue to obtain seabuckthorn leaf powder, which is set aside;

[0048] 4. Extraction

[0049] According to the mass ratio of methanol extract: seabuckthorn leaf powder = 5:1, use the methanol extract to reflux extract the seabuckthorn leaf powder, and reflux twice; after the reflux extraction, the methanol is recovered under r...

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PUM

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Abstract

The invention discloses a method for extracting hypoglycemic components from seabuckthorn leaves, which belongs to the technical field of extracting plant hypoglycemic components; the technical problem to be solved is the difficulty in extracting hypoglycemic components from seabuckthorn leaves; The method for extracting the hypoglycemic component follows the following steps: after the leaves of seabuckthorn are de-enzymed and dried, they are crushed into coarse powder, added with water 10-20 times the weight of the coarse powder, boiled, and cooled to room temperature to obtain pre-boiled sea-buckthorn leaves; Add 1-5wt% Daqu to the quality of the cooking liquid, mix well, and keep warm at 26-36°C for 3-5 days; filter the pre-boiling liquid after soaking in heat preservation, and dry the filter residue to obtain seabuckthorn leaf powder, which is set aside; extract according to the mass ratio Solution: Seabuckthorn leaf powder = 5:1 Take the extract and carry out reflux extraction on the seabuckthorn leaf powder for 2-3 times; after reflux extraction, recover the extract under reduced pressure to obtain a paste-like seabuckthorn leaf extract.

Description

technical field [0001] The invention discloses a method for extracting hypoglycemic components from seabuckthorn leaves, which belongs to the technical field of extraction of plant hypoglycemic components. Background technique [0002] Corosolic acid, also known as 2α-hydroxyursolic acid, is a pentacyclic triterpenoid acid derived from the extract of Lagerstroemia grandis. Garcia (1940) fed ordinary rabbits with dry leaves of Lagerstroemia indica and found that the blood sugar of the rabbits decreased. In 1956, he used the extract of Lagerstroemia indica leaves to conduct clinical experiments and found that it had the effect of lowering blood sugar in diabetic patients. Murakami (1993) isolated two kinds of triterpene acids (corosolic acid and maslinic acid) from the leaves of Lagerstroemia grandis, and found that corosolic acid has the effect of lowering blood sugar. Hamamoto (1999) found that mice and rabbits had obvious hypoglycemic effect after taking corosolic acid, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P3/10
Inventor 张小民郭海利张吉科林美珍姚莹
Owner SHANXI JINKEHAI BIOLOGICAL PROD
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