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Method for online separation and purification of butanol, acetone and ethanol in fermentation solution by using active carbon in-situ adsorption

A technology of activated carbon and fermentation broth, which is applied in the biological field to achieve the effects of low product purity, strong adsorption capacity and energy saving

Active Publication Date: 2014-12-31
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no reports and related patents on the simultaneous separation and purification of butanol, acetone and ethanol using activated carbon in-situ adsorption method coupled fermentation equipment

Method used

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  • Method for online separation and purification of butanol, acetone and ethanol in fermentation solution by using active carbon in-situ adsorption
  • Method for online separation and purification of butanol, acetone and ethanol in fermentation solution by using active carbon in-situ adsorption
  • Method for online separation and purification of butanol, acetone and ethanol in fermentation solution by using active carbon in-situ adsorption

Examples

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Embodiment 1

[0047] A method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by using activated carbon in-situ adsorption;

[0048] ① if figure 1 As shown, the seed medium was first passed nitrogen in the seed tank for 10 minutes to deoxygenate, then sterilized at 121°C for 30 minutes, cooled to room temperature, inserted into the acetone butanol ethanol production bacteria, and the production bacteria were cultivated to the most active logarithm of growth growth period. The cultivation time until the logarithmic growth phase is 12-18 hours, preferably 15 hours; the cultivation temperature is 30-40°C, preferably 37°C. After the seed culture is completed, it is ready to be inserted into the fermenter.

[0049] ② if figure 1 As shown, first sterilize the fermentation medium at 121°C for 30 minutes, then pass nitrogen gas for 2 hours to deoxygenate, cool to room temperature, turn on the pump I, and put the seed liquid containing acetone, buta...

Embodiment 2

[0054] A method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by using activated carbon in-situ adsorption;

[0055] The difference with embodiment 1 is:

[0056] When the pH of the fermentation broth is lower than 5.0, ammonia water is automatically added to adjust the pH to above 5.0.

[0057] The experimental data are shown in Table 1 and Table 2.

[0058] Summary: After the fermentation, the concentrations of butanol and ABE total solvent were 2.4% (w / v) and 4.0% (w / v), respectively, which were 71.4% and 73.9% higher than those in Comparative Example 1. Glucose consumption was 12.0%, an increase of 71.4% compared with Comparative Example 1. The concentrations of butanol and ABE total solvent recovered after desorption were 11.2% (w / v) and 14.9% (w / v), which were 8.0 times and 6.5 times higher than those in Comparative Example 1, respectively. The production intensity of butanol and ABE total solvent is 0.26g / L / h and 0.43g / ...

Embodiment 3

[0060] A method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by using activated carbon in-situ adsorption;

[0061] The difference with embodiment 1 is:

[0062] When the pH of the fermentation broth is lower than 5.0, ammonia water is automatically added to adjust the pH to above 5.0. The fermentation method is feed-fed fermentation. When the glucose concentration in the fermentation broth drops below 10g / L, glucose is added to continue to provide carbon sources for the fermentation until the fermentation is terminated.

[0063] The experimental data are shown in Table 1 and Table 2.

[0064] Summary: After the fermentation, the concentrations of butanol and ABE total solvent were 3.3% (w / v) and 5.4% (w / v), respectively, which were 36.4% and 33.3% higher than those in Comparative Example 1. Glucose consumption was 16.5%, which was 2.4 times higher than that of Comparative Example 1. The concentrations of butanol and ABE tota...

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Abstract

The invention provides a method for online separation and purification of butanol, acetone and ethanol in a fermentation solution by using active carbon in-situ adsorption, and belongs to the field of a biotechnology. The method comprises the following steps of: (1) culturing acetone, butanol and ethanol production strains; (2) fermenting and carrying out online in-situ adsorption on acetone, butanol and ethanol by utilizing activated carbon; and (3) after the adsorption, carrying out desorption, and condensing and recycling to obtain butanol, acetone and ethanol crude products. The method has the beneficial effects of high fermentation efficiency and low separation and purification costs.

Description

technical field [0001] The invention relates to a method for on-line separation and purification of butanol, acetone and ethanol in fermentation broth by using active carbon in-situ adsorption, and belongs to the field of biotechnology. Background technique [0002] Butanol is an important liquid energy source and chemical, which can be obtained through microbial fermentation, usually using acetone-butanol-ethanol (ABE) fermentation to produce butanol, and at the same time produce acetone and ethanol, as well as a small amount of organic acids. (See non-patent literature for details: Kumar, M, Gayen, K..Developments in biobutanol production: new insights.Applied Energy, 88:1999-2012, 2011). However, the concentration of butanol at the end point in the fermentation broth is usually 1.0 to 2.0 % (w / v), the concentration of acetone is 0.5-1.0% (w / v). In addition, the boiling point of butanol is 117.7°C, which is 100°C higher than the boiling point of water. Therefore, if the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/16C12P7/28C12P7/06C07C29/76C07C31/12C07C31/08C07C45/79C07C49/08C12R1/145C12R1/19
CPCY02E50/17Y02E50/10
Inventor 薛闯杨尚天白凤武
Owner DALIAN UNIV OF TECH
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