Method for high-salt screening of saline-alkali resistant rosa chinensis plant

A salinity-tolerant, rose technology, applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of unsuitable rose growth and high soil salinity.

Inactive Publication Date: 2013-04-03
王昱蓉 +1
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Problems solved by technology

Although Tianjin is located on the shore of the Bohai Sea, the soil is also salinized to a certain extent, but the soil in Golmud has a higher salinity, which is not suitable for the growth of roses.

Method used

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  • Method for high-salt screening of saline-alkali resistant rosa chinensis plant
  • Method for high-salt screening of saline-alkali resistant rosa chinensis plant
  • Method for high-salt screening of saline-alkali resistant rosa chinensis plant

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Embodiment

[0044]Using Chinese roses as materials, the roses suitable for normal growth in saline-alkali land are selected through high-salt screening. The screening process is as follows:

[0045] (1) Preparation of Chinese rose explant cultivation medium

[0046] Vermiculite, perlite and nutrient soil are prepared in a mass ratio of 10:2:1, and 1 cm of river sand is laminated on the substrate, sterilized with 0.5% potassium permanganate aqueous solution, and then 0.3% rooting liquid Spray the substrate. Vermiculite and perlite are mainly for breathability and strong moisture retention, and provide a good growth condition for plants; press about 1 cm of river sand, mainly for fixation.

[0047] (2) Preparation of rose explants

[0048] Select thicker lignofibrous branches of vascular bundles, cut into stem segments of about 10 cm, and leave 2-3 leaves for each stem segment. Soak the morphological lower end of rose stems in 3% rooting solution for 1 hour, insert the cuttings into ...

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Abstract

The invention relates to a method for high-salt screening of a saline-alkali resistant rosa chinensis plant. In particular, rosa chinensis which is planted in Tibet plateau is obtained by the method for high-salt screening of saline-alkali resistant rosa chinensis. The method comprises the steps of inducing obtained rosa chinensis explant culture medium suitable for rosa chinensis explants out of a sprout texture and then irrigating and screening by a high-salt culture medium to obtain the saline-alkali resistant rosa chinensis; cutting a fibroid branch with thick vascular bundle into 10cm stem sections, sterilizing the culture mediums by 0.5% potassium permanganate according to the ratio of vermiculite to perlite to nutrient soil being 10:2:1, and then spraying the medium by 0.3% rooting liquid, wherein high-salt screening is diluting and irrigating by sodium chloride liquid culture mediums B with different concentrations, and selecting the plant which can normally grow. By adopting the method, the growth boundaries of fresh flowers are broken through; five saline-alkali resistant rosa chinensises can be obtained from genetic modification and individual gene screening, and a blank no fresh flower is available in golmud is filled.

Description

technical field [0001] The invention relates to a method for high-salt screening of saline-alkali-tolerant rose plants, in particular to obtaining surviving roses planted on the Qinghai-Tibet Plateau by using the method for high-salt screening of saline-alkali-tolerant roses. Background technique [0002] Rose (Rosa chinensis) belongs to the Rosaceae family. It is an evergreen or deciduous shrub. It is known as the queen of flowers, also known as "Moon Moon Red". The natural flowering period is from May to November. Tianjin is known as the “Hometown of Rose” and has a long history of rose cultivation. After Tianjin designated rose as the city flower in 1984, the production and cultivation of rose have developed greatly. Friendship, Prosperity, Development". However, Golmud City, which is located at an altitude of 2,900 meters on the Qinghai-Tibet Plateau, does not grow flowers. Not only do people here have no flowers in their lives, but even grand celebrations and meetings...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 王昱蓉季静
Owner 王昱蓉
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