Targeting polypeptide-gene composite as well as preparation method and application of composite

A targeted and complex technology, applied in the field of biomedicine, can solve problems such as nerve cell damage and accelerate central nervous system diseases, achieve the effect of inhibiting activation, inhibiting occurrence or deterioration, and good development and application prospects

Inactive Publication Date: 2013-04-03
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Abstract
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Problems solved by technology

However, studies have shown that overactivated microglia may accelerate the process of some central nervous system diseases, and the cytotoxic factors released by them will also cause further damage to nerve cells

Method used

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  • Targeting polypeptide-gene composite as well as preparation method and application of composite
  • Targeting polypeptide-gene composite as well as preparation method and application of composite
  • Targeting polypeptide-gene composite as well as preparation method and application of composite

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Embodiment Construction

[0021] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0022] 1. Preparation of targeted peptide-gene complexes

[0023] 1. Preparation of fusion peptide HIV-Tat49-57 / V9 of HIV-Tat49-57 peptide and V9 peptide

[0024] The fusion peptide HIV-Tat49-57 / V9 is formed by directly linking the carboxyl terminus of the HIV-Tat49-57 peptide and the amino terminus of the V9 peptide, and the amino acid sequence is RKKRRQRRLTQQMKFVV (SEQ ID No.1).

[0025] The fusion peptide HIV...

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Abstract

The invention discloses a targeting polypeptide-gene composite. The composite is formed by a binding fusion protein of HIV-Tat (49-57) peptide (RKKRRQRRR) (Human Immunodeficiency Virus Trans-activator of Transcription) and V9 peptide (LTQQMKFVV) and an HIF-1alphashRNA (Hypoxia Inducible Factor-1alpha Short Hairpin Ribonucleic Acid) carrier by means of electrostatic interaction; the composite can use the guide effect of the V9 (CX3C-chemokine receptor 1) receptor on the surface of microglia, and, at the same time when the V9 peptide and the CX3CR1 receptor act with each other, the HIF-1alphashRNA is efficiently transfected into the microglia to interfere with the HIF-1alpha (Hypoxia Inducible Factor-1alpha) expression and inhibit the microglia activation so as to further inhibit the occurrence and progression of a microglia-mediated central nervous system disease. The composite can be applied to the preparation of a medicine for treating the microglia-mediated central nervous system disease and has good development and application prospect in the field of treating the nervous system disease.

Description

technical field [0001] The invention belongs to the field of biomedicine, relates to a polypeptide-gene complex, and also relates to a preparation method of the complex and an application in the field of pharmacy. Background technique [0002] As the first line of defense in the central nervous system, microglia play an important role in the inflammatory response. When foreign pathogens invade or cells die, microglia are quickly activated and eliminated, and at the same time release inflammatory factors to mediate the inflammatory response. However, studies have shown that overactivated microglia may accelerate the process of some central nervous system diseases, and the cytotoxic factors released by them will also cause further damage to nerve cells. Therefore, appropriately inhibiting the activity of microglia may be an effective way to treat central nervous system diseases. [0003] Hypoxia-inducible factor-1α (HIF-1α) is the only nuclear transcription factor discovered...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K31/7088A61K47/42A61P25/28C12N15/66
Inventor 杨曌李飞胡荣储卫华冯华
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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