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Method for measuring content of benzo-a-pyrene in mainstream smoke of cigarettes

A technology of cigarette mainstream smoke and determination method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of good sensitivity and repeatability, easy operation, selectivity and sensitivity improvement

Inactive Publication Date: 2013-04-24
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, some foreign scholars use LC-APPI-MS / MS to determine 10 kinds of carcinogenic polycyclic aromatic hydrocarbons in cigarette mainstream smoke, but the pretreatment is more complicated, including solvent extraction and solid phase extraction; - MS / MS determination of B[a]P in mainstream cigarette smoke has not been reported yet

Method used

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  • Method for measuring content of benzo-a-pyrene in mainstream smoke of cigarettes
  • Method for measuring content of benzo-a-pyrene in mainstream smoke of cigarettes

Examples

Experimental program
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example 1

[0044] 1. Instruments and reagents:

[0045] BaP standard (purity ≥98%), deuterated BaP (d12-BaP, purity ≥98%) were purchased from Supelco, USA; silica gel column (500 mg, 3 mL) was purchased from Agilent; acetonitrile (TEDIA company Inc ., Ohio, USA), all solvents were chromatographically pure, and the water used in the experiment was deionized water.

[0046] Agilent 1200 Ultra-Resolution Liquid Chromatograph (Autosampler G1367D, Binary Solvent Pump G1312B, Column Compartment G1316B) (Agilent Technologies, Palo Alto, CA). API 5500 triple quadrupole tandem mass spectrometry system (Applied Biosystems, Foster City, CA) equipped with a photoionization ionization source (APPI). Data acquisition and processing were implemented on Analyst 1.5.1 software (Applied Biosystems). Hitachi L-2130 pump (Hitachi High-Tech Corporation, Japan), Cerulean SM450 linear smoking machine (Cerulean, Ltd.).

[0047] 2. Sample handling:

[0048] Filter collection: according to GB / T 16450, the sa...

example 2

[0051] Example 2: As described in Example 1, for three different brands of cigarette samples. The average content levels of B[a]P in mainstream smoke of three brands of cigarettes were 6.20 ng / cigarette, 9.88 ng / cigarette and 10.1 ng / cigarette, respectively.

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Abstract

The invention relates to a method for measuring content of benzo-a-pyrene in mainstream smoke of cigarettes, which is characterized by comprising the following steps of: catching mainstream smoke by adopting a Cambridge filter; carrying out cyclohexane oscillation extraction on a sample; carrying out solid-phase extraction on extraction liquid by a silica gel column; collecting filtrate and eluent; carrying out rotary evaporation at a temperature of 40 DEG C to a nearly dry state; redissolving the obtained product into 1mL of acetonitrile; introducing a liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometer to carry out measurement so as to accurately detect the content level of the benzo-a-pyrene in the mainstream smoke of the cigarettes. According to the invention, due to adoption of a manner of using a perylene standard substance as an internal standard for qualifying analyte, an error caused in the process of preprocessing the sample can be reduced; tandem mass spectrometry further improves selectivity and accuracy of the method; and an atmospheric pressure photoionization ion source can well ionize a benzo-a-pyrene compound with low polarity. For a cigarette smoke sample with a complex matrix, a dopant, a tandem mass spectrum and the perylene internal standard are quantitatively used, so that selectivity and sensitivity of the method can be effectively improved.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of physical and chemical testing of cigarette mainstream smoke samples, and mainly relates to the technical field of determination of polycyclic aromatic hydrocarbons in cigarette mainstream smoke, specifically a liquid chromatography-photoionization-tandem mass spectrometer (LC-APPI) - MS / MS) for the determination of B[a]P content in mainstream cigarette smoke. Background technique: [0003] B[a]P is one of more than 20 polycyclic aromatic hydrocarbons known to be carcinogenic, accounting for 1%-20% of all polycyclic aromatic hydrocarbons in the environment, and is the most representative internationally recognized strong carcinogen. B[a]P can be absorbed by the human body through the skin, respiratory tract, digestive tract, etc., and the products of metabolic activation in the body can cause tumors in the liver, skin, lungs, etc., and have certain toxicity to the reproductive system and immun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 侯宏卫张小涛田永峰刘彤陈欢韩书磊胡清源
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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