Enzyme biological fuel cell positive pole and preparation method and application thereof

A biofuel cell and anode technology, applied in biochemical fuel cells, battery electrodes, circuits, etc., can solve the problems of affecting performance, prone to agglomeration, poor dispersion, etc. Effect

Inactive Publication Date: 2013-04-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although carbon nanotubes will improve the performance of enzyme electrodes, there is a problem in the application of carbon nanotubes, that is, the hydrophobic surface of carbon nanotubes makes it poorly dispersed in water, and it is easy to agglomerate. The chemical binding site also affects its performance

Method used

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  • Enzyme biological fuel cell positive pole and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] I, preparation of enzyme biofuel cell bioanode Gel1 / CNT / BSA / GOD:

[0045] (1) Take a certain amount of short multi-walled carbon nanotubes, with a diameter of 20-30nm and a length of 0.5-2μm, soak in acetone for 30 minutes, then filter, wash with distilled water, and dry. Then the cleaned carbon nanotubes were added with H 2 SO 4 / HNO 3 (3:1, volume ratio) in mixed acid solution at 30°C, 100Hz ultrasonic for 6h, filtered, cleaned with distilled water, dried in vacuum, and set aside.

[0046] (2) Take 4mg of carbon nanotubes treated in step (1), add 1ml of 0.1% by mass gelatin aqueous solution, and ultrasonicate at 40°C for 6 hours at 100Hz to obtain a multi-walled carbon nanotube gelatin composite dispersion.

[0047] (3) Take carbon paper with a size of 1×1 cm, soak it in acetone solution for 30 minutes, then wash it in distilled water, and dry it in a vacuum oven at 100°C for 12 hours under vacuum.

[0048] (4) Evenly drop 1ml of the multi-walled carbon nanotube g...

Embodiment 2

[0054] I, preparation of enzyme biofuel cell bioanode Gel3 / CNT / BSA / GOD:

[0055] (1) Same as step I (1) of Example 1.

[0056] (2) Take 4 mg of carbon nanotubes treated in step (1), add 1 ml of 0.3% by mass gelatin aqueous solution, and sonicate at 40° C. for 6 hours to obtain a multi-walled carbon nanotube gelatin composite dispersion.

[0057] (3) Same as step I (3) of Example 1.

[0058] (4) Evenly drop 1ml of the multi-walled carbon nanotube gelatin composite material dispersion prepared in step (2) onto the surface of the carbon paper cleaned in step (3), and dry.

[0059] (5) Take 1ml of BSA / GOD mixture (wherein, the final concentration of BSA is 1% by mass, and the ratio of BSA and GOD is 1:1 by mass) and drop it on the surface of the carbon paper treated in step (4), Add 0.1ml of 0.3% by volume glutaraldehyde dropwise, place it at 4°C for 12h, and rinse off excess substances with distilled water. Connect the silver wire to the carbon paper to obtain the enzyme bioan...

Embodiment 3

[0062] I, preparation of enzyme biofuel cell bioanode Gel32 / CNT / BSA / GOD:

[0063] (1) Same as step I (1) of Example 1.

[0064] (2) Take 2 mg of carbon nanotubes treated in step (1), add 1 ml of 0.3% by mass gelatin aqueous solution, and sonicate at 40°C for 6 hours to obtain a multi-walled carbon nanotube gelatin composite dispersion.

[0065] (3) Same as step I (3) of Example 1.

[0066] (4) Evenly drop 1ml of the multi-walled carbon nanotube gelatin composite material dispersion prepared in step (2) onto the surface of the carbon paper cleaned in step (3), and dry.

[0067] (5) Take 1ml of BSA / GOD mixture (wherein, the final concentration of BSA is 1% by mass, and the ratio of BSA and GOD is 1:1 by mass) and drop it on the surface of the carbon paper treated in step (4), Add 0.1ml of 0.3% by volume glutaraldehyde dropwise, place it at 4°C for 12h, and rinse off excess substances with distilled water. Connect the silver wire to the carbon paper to obtain the enzyme bioano...

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Abstract

The invention discloses an enzyme biological fuel cell positive pole and a preparation method and application thereof. Carbon paper is served as an electrode substrate, gelatin is served as modified material of a carbon nano tube, the carbon nano tube with the gelatin modified and bovine serum albumin are served as enzyme carriers, enzyme is served as catalyst, glutaraldehyde is served as cross-linking agent, and therefore the enzyme biological fuel cell positive pole is combined. The preparation method is simple and effective, convenient to operate, environment-friendly and low in cost. Due to the fact that the gelatin can successfully solve the problem that the carbon nano tube is not good in dispersibility in water solution, the enzyme biological fuel cell positive pole which is provided by the preparation method can greatly improve power density of the enzyme biological fuel cell positive pole. The enzyme biological fuel cell positive pole can be used widely, not only in preparation of biological electrodes of enzyme biological fuel cells, but also in preparation of electrodes of biological sensors.

Description

technical field [0001] The invention belongs to the field of microbial fuel cell biotechnology, and in particular relates to an anode of an enzyme biofuel cell and a preparation method and application thereof. Background technique [0002] The development of biofuel cells utilizing microbial metabolic processes combined with electrode reactions provides a new avenue for renewable energy production and waste treatment. A biofuel cell is a device that uses microorganisms as the main body of the reaction to directly convert the chemical energy of fuel (organic matter) into electrical energy. It is a device that uses various organisms as catalysts. Its type is determined by the type of biocatalyst decided. Biofuel cells with microorganisms as catalysts use living cells to catalyze the oxidation of fuel, but enzyme biofuel cells with enzymes as catalysts use enzymes for this purpose. At present, the advantage of biofuel cells using microorganisms as catalysts is that the batter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01M4/88H01M4/86H01M8/16
CPCY02E60/527Y02E60/50
Inventor 朴金花吕稳张秀花姜建国
Owner SOUTH CHINA UNIV OF TECH
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