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Preparation method of multifunctional mesoporous directly-cladded fluorescence nano-bioprobe

A nano-biological probe and multi-functional technology, which is applied in the field of preparation of fluorescent multi-functional nano-biological probes, can solve problems such as numerous procedures, long cycle times, multi-functional nano-probe morphology, and stability effects, and achieve simplified synthesis The process and preparation process are simple and easy to achieve

Active Publication Date: 2014-07-09
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the preparation method of mesoporous fluorescent multifunctional nano-biological probes is mostly a "two-step method", that is, a layer of non-porous silicon is grown on the surface of the nanocrystal, and then mesoporous silicon is grown using this as a nucleus, but the above-mentioned method has many steps , longer period
In addition, it is difficult to control the dispersion of nanoparticles during the reaction process, so the morphology and stability of multifunctional nanoprobes are easily affected.

Method used

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  • Preparation method of multifunctional mesoporous directly-cladded fluorescence nano-bioprobe
  • Preparation method of multifunctional mesoporous directly-cladded fluorescence nano-bioprobe
  • Preparation method of multifunctional mesoporous directly-cladded fluorescence nano-bioprobe

Examples

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Embodiment 1

[0043] This embodiment provides a method for preparing a fluorescent multifunctional nano-biological probe directly coated with mesoporous, which includes the following steps:

[0044] (1) Convert hydrophobic up-conversion nanocrystals to hydrophilic to form the first dispersion;

[0045] (2) Fully mix deionized water, ethanol and ammonia water to form a second dispersion, add the first dispersion to the second dispersion and heat;

[0046] (3) adding a silicon source to the mixed solution of the first and second dispersion liquids to form a mesoporous fluorescent multifunctional nano-spherical product;

[0047] (4) The template agent (hexadecyltrimethylammonium bromide) was removed from the nano-spherical product to prepare a fluorescent multifunctional nano-biological probe directly coated with mesoporous pores.

[0048] The step (1) specifically includes the following steps:

[0049] (1.1) Prepare 10-15 mg of hydrophobic NaYF with a particle size of 30-60 nm 4 : Yb, Er n...

Embodiment 2

[0071] This example provides a method for preparing a mesoporous fluorescent multifunctional nano-biological probe, the basic steps of which are the same as in Example 1, except that the following specific steps are different:

[0072] (1) Prepare 10 mg of hydrophobic NaYF with a particle size of 30-60 nm 4 : Yb, Er nano-dispersed in cyclohexane to form the first mixed solution; prepare 100 mg CTAB and 20 mL deionized water, mix and dissolve CATB and deionized water to form the second mixed solution; Mix the two mixtures and stir thoroughly for 3-5 hours to obtain the third mixture; heat the third mixture to 60-85°C and keep it for 40-80 minutes to evaporate and remove cyclohexane, and cool to obtain a transparent aqueous solution, that is, the first dispersion ,spare;

[0073] (2) Prepare 2 mL of ammonia water, 40 mL of deionized water and 75 mL of ethanol; mix the three solutions evenly to obtain the second dispersion;

[0074] (3) Prepare 80 μL of TEOS, dissolve it in 5 m...

Embodiment 3

[0077] This embodiment provides a method for preparing a mesoporous fluorescent multifunctional nano-biological probe. The basic steps are the same as in Example 1, except that the following specific steps are different:

[0078] (1) Prepare 10 mg of hydrophobic NaYF with a particle size of 30-60 nm 4 : Yb, Er nano-dispersed in cyclohexane to form the first mixed solution; prepare 100 mg CTAB and 20 mL deionized water, mix and dissolve CATB and deionized water to form the second mixed solution; Mix the two mixtures and stir thoroughly for 3-5 hours to obtain the third mixture; heat the third mixture to 60-85°C and keep it for 40-80 minutes to evaporate and remove cyclohexane, and cool to obtain a transparent aqueous solution, that is, the first dispersion ,spare;

[0079] (2) Prepare 1mL ammonia water, 10mL deionized water and 35mL ethanol; mix the three solutions evenly to prepare the second dispersion;

[0080] (3) Prepare 30 μL of TEOS, dissolve it in 5 mL of ethanol solu...

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Abstract

The invention discloses a preparation method of a multifunctional mesoporous directly-cladded fluorescence nano-bioprobe. The method comprises the following steps: firstly, rare earth doped with up-conversion luminescence nanocrystallines is taken as a nucleus, hydrophobic up-conversion nanocrystallines are converted into hydrophilic up-conversion nanocrystallines by a cationic surface active agent (hexadecyl trimethyl ammonium bromide); then, the hydrophilic up-conversion nanocrystallines are dispersed in a mixed solution of water, ethanol and ammonia water; a silicon source is added dropwise under an alkaline condition subsequently, hydrolyzed and self-assembled to the surfaces of the up-conversion nanocrystallines under the electrostatic reaction, so that multifunctional mesoporous fluorescence nanospheres are formed; and finally, a template agent is removed, so that the multifunctional mesoporous directly-cladded fluorescence nano-bioprobe is obtained. According to the method, the preparation process is simpler, the production efficiency is higher, and the compounded mesoporous nano spherical material not only is uniform in size and stable in structure, but also has the advantages of higher upconversion fluorescence intensity, larger specific surface area and the like, and can be applied to bio-medical fields such as upconversion fluorescence imaging, targeting drug loading and the like.

Description

technical field [0001] The invention belongs to the technical field of nano-biological materials, and in particular relates to a method for preparing a fluorescent multi-functional nano-biological probe directly coated with mesoporous pores. Background technique [0002] The combination and assembly of different types of nanofunctional materials can well promote the development of multifunctional nanoplatforms for multimodal biological imaging and therapy. With the development of nanotechnology, multifunctional nanobiological probes with characteristics such as fluorescence and mesoporous structure have received unprecedented attention. Fluorescent materials and mesoporous materials have broad application prospects in immune analysis, drug release, in vivo imaging, and cancer cell physiotherapy, and are important application materials for in-depth research in the field of biomedicine. Multifunctional mesoporous nanomaterials have many advantages. On the one hand, biological...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/85C09K11/02
Inventor 孙丽宁施利毅刘涛仇衍楠王志娟
Owner SHANGHAI UNIV