Method for identifying glyphosate resistant cotton rapidly

A technology of anti-glyphosate and identification method, which is applied in horticultural methods, botanical equipment and methods, seed and rhizome treatment, etc., can solve the problems of expensive molecular detection instruments and reagents, occupy a large space, and be difficult to carry out, and achieve results The effect of good consistency, small space occupation and small workload

Inactive Publication Date: 2014-12-31
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

This method is intuitive and accurate, but it takes a long time, takes up a lot of space, and has strong seasonality
Molecular and protein assays are also a method to identify transgenic glyphosate-resistant cotton, but due to the expensive instruments and reagents required for molecular assays and high professional technical requirements for inspectors, it is difficult for most companies and research institutes to carry out. In addition, At present, domestic glyphosate-resistant cotton has not yet been commercialized, foreign genes have not been published, and molecular detection methods are limited

Method used

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  • Method for identifying glyphosate resistant cotton rapidly
  • Method for identifying glyphosate resistant cotton rapidly

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040]Glyphosate-resistant cotton materials (KCM1, KCM2, KCM3) and non-glyphosate-resistant cotton materials (Zhongmian Institute 49, Zhongmian Institute 79) were used at concentrations of 4.1ga.i. / L and 6.15ga.i. / L and 8.2ga.i. / L glyphosate solution soaked for 1 hour and 2 hours, the experimental results are listed in Table 1.

[0041] Table 1 The emergence rate and radicle growth inhibition rate of cotton seeds to be tested after being treated with different concentrations of glyphosate for different times

[0042]

[0043] The negative value of the radicle growth inhibition rate indicated that the radicle growth of the glyphosate treatment group was greater than that of the water control group, indicating that the glyphosate treatment had no effect on the radicle growth.

[0044] It can be seen from Table 1 that after soaking the tested cotton seeds in 4.1g a.i. / L glyphosate solution for 1-2 hours, the emergence rate of the three glyphosate-resistant cottons was 82.2%-9...

Embodiment 2

[0049] Glyphosate-resistant cotton materials (KCM4, KCM5, KCM6, KCM7, KCM8) and non-glyphosate-resistant cotton materials (China Cotton Institute 41, China Cotton Institute 43, China Cotton Institute 52, China Cotton Institute 63, and China Cotton Institute 69 ), soaked with 8.2g a.i. / L glyphosate solution for 1 hour, and the experimental results are listed in Table 2.

[0050] Seed emergence rate, radicle length and radicle growth inhibition rate of cotton seeds to be tested after table 2 treatment

[0051]

[0052] According to the data in Table 2, it can be seen that for non-glyphosate-resistant cotton materials, glyphosate treatment significantly affected the emergence rate (the emergence rate decreased from 72.2%-92.2% to 4.4%-14.4%), and the radicle growth inhibition rate was as high as 75.5%-81.3%; while for glyphosate-resistant cotton materials, glyphosate treatment had little effect on the emergence rate (the emergence rate decreased from 95.6%-100% to 75.6%-93.3%)...

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Abstract

A method for identifying glyphosate resistant cotton rapidly includes that cotton seeds to be tested are soaked in glyphosate solution, wherein white color is exposed on the cotton seeds already; the soaked cotton seeds are rinsed with clear water and then placed in an illumination incubator for sand culture; the number of emerged seedlings is counted, and the emergence rate is obtained through calculation; and whether the cotton to be tested is the glyphosate resistant cotton is judged according to the emergence rate. Through the method for identifying the glyphosate resistant cotton rapidly, the defects that a field identification method or a greenhouse identification method is long in cycle, heavy in workload, prone to be restricted by seasons and the like can be overcome, and the method for identifying the glyphosate resistant cotton rapidly has high practicability and operability.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a herbicide bioassay technology, and in particular to a rapid identification method for glyphosate-resistant cotton. Background technique [0002] Cotton is an important economic crop in my country, with an annual planting area of ​​5 million hectares 2 , accounting for 26% of the total area of ​​commercial crops, is an important pillar industry of the national economy. However, weeds in my country's cotton fields are more harmful, causing losses of 14% to 16% per year, which seriously restricts the high-quality and efficient production of cotton. As a broad-spectrum systemic herbicide, glyphosate plays an important role in the control of weeds in cotton fields. However, since glyphosate has no selectivity between weeds and cotton, it is easy to cause phytotoxicity and even death to conventional cotton varieties when glyphosate is used. [0003] The application of tra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01G31/00A01C1/00
Inventor 马艳马小艳崔金杰姜伟丽雒珺瑜马亚杰奚建平李希风
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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