Artificial skin graft for genipin immobilized activin B and preparation method thereof
An artificial skin and graft technology, applied in the field of artificial skin grafts and preparation, can solve the problems of affecting wound care effect, reduced water retention capacity, lack of durability, etc. Compatibility effect
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Embodiment 1
[0022] A preparation method for an artificial skin graft of genipin-fixed activin B, comprising the following steps:
[0023] 1) Raw material purification:
[0024] Weigh 5g gelatin and dissolve it in 100mL distilled water, heat to 50°C and stir to dissolve;
[0025] Weigh 10g carboxymethyl chitosan and dissolve in 100mL distilled water, stir to dissolve at room temperature;
[0026] The dissolved gelatin and carboxymethyl chitosan solutions were centrifuged at a high speed of 15,000 rpm for 5 minutes, separated to obtain supernatants, and freeze-dried for 24 hours to obtain purified gelatin and carboxymethyl chitosan respectively;
[0027] 2) Preparation of artificial skin grafts with genipin-immobilized Activin B growth factor
[0028] Weigh 2.25g of purified gelatin and 0.25g of carboxymethyl chitosan respectively, sterilize them by ultraviolet light for 30min, then add them into sterilized distilled water, heat to 50°C, stir to dissolve and cool to room temperature, then...
Embodiment 2
[0032] A preparation method for an artificial skin graft of genipin-fixed activin B, comprising the following steps:
[0033] 1) Purify gelatin and carboxymethyl chitosan according to the method in Example 1;
[0034] 2) Preparation of artificial skin grafts:
[0035] Weigh 4.5g of purified gelatin and 0.5g of carboxymethyl chitosan respectively, sterilize by ultraviolet light for 30min, then add them into sterilized distilled water, heat to 50°C, stir to dissolve and cool to room temperature, and set Make up to 40mL to get gelatin, carboxymethyl chitosan mixture;
[0036] Weigh 25 mg of genipin and dissolve it in 10 mL of sterilized distilled water, filter and sterilize to obtain an aqueous solution of genipin;
[0037] Pour the genipin aqueous solution into the mixture of gelatin and carboxymethyl chitosan, stir well at room temperature, add 50 μL of 10 μg / mL activin B solution, stir well, pour it into a mold, and place it at 4°C to form a gel , to obtain genipin-immobili...
Embodiment 3
[0039] A preparation method for an artificial skin graft of genipin-fixed activin B, comprising the following steps:
[0040] 1) Purify gelatin and carboxymethyl chitosan according to the method in Example 1;
[0041] 2) Preparation of artificial skin grafts:
[0042] Weigh 9g of purified gelatin and 1g of carboxymethyl chitosan respectively, sterilize them by ultraviolet light for 30min, then add them into sterilized distilled water, heat to 50°C and stir to dissolve, then cool to room temperature, and make up to volume with sterilized distilled water to 40mL to obtain gelatin, carboxymethyl chitosan mixture;
[0043] Weigh 25 mg of genipin and dissolve it in 10 mL of sterilized distilled water, filter and sterilize to obtain an aqueous solution of genipin;
[0044] Pour the genipin aqueous solution into the mixture of gelatin and carboxymethyl chitosan, stir well at room temperature, add 50 μL of 10 μg / mL activin B solution, stir well, pour it into a mold, and place it at ...
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