One-stop extraction method of bioactive substances in crayfish by-products

Abstract

The invention relates to a coordinated process extraction method of biological active substances in crayfish by-products, belonging to the field of a biological active substance extraction method. The coordinated process extraction method comprises the following steps of: sequentially carrying out enzymolysis treatment on crushed crayfish shells and crayfish heads which are obtained by boiling through water and removing crayfish meat, and taken as raw materials; spraying and drying a filtered solution to obtain hydrolyzed protein powder; carrying out organic acid leaching treatment on a filtered solid, and spraying and drying the filtered solution to obtain organic calcium; adding the filtered solid into ethanol to be treated; carrying out rotary evaporation on the filtered solution to obtain an astaxanthin rough product; and finally, carrying out hydrogen peroxide immersion de-coloring treatment on the filtered solid to obtain de-colored chitin. According to the coordinated process extraction method disclosed by the invention, the crushed crayfish shells and crayfish heads, which are obtained by boiling through the water and removing the crayfish meat, are taken as the raw materials, and a scientific and simple process route is utilized for sequentially obtaining the food-grade standard hydrolyzed protein powder, organic calcium, astaxanthin and chitin products; a production period is shortened, the production efficiency is improved and a product additional value is obviously improved; and a production process is clean production, wastewater emission is reduced and the coordinated process extraction method is good for environmental protection.

Description

Technical field: [0001] The invention relates to a one-stop extraction method of bioactive substances in crayfish by-products, belonging to the field of bioactive substance extraction methods. technical background: [0002] Shrimp head and shell are the main by-products of crayfish, accounting for 70% to 80% of the shrimp's weight, rich in protein, calcium, chitin and its unique astaxanthin. At present, the utilization of crayfish by-products is concentrated in the direction of extracting chitin. The traditional method of extracting chitin is the acid-base method. Generally, 4% to 6% dilute hydrochloric acid is used to soak the shrimp shells first to make the carbonates of calcium and magnesium change. The hydrochloride is dissolved in water, and because its main component is calcium carbonate, it is also called decalcification reaction. Utilizing the characteristics that protein dissolves quickly in alkali and chitin degrades little in alkali, use 8% to 10% dilute alkali t...

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Problems solved by technology

[0004] The invention aims at the use of strong acid and strong alkali in the traditional process to destroy the molecular structure of chitin, and the waste liquid pollutes the environment, the cost of sewage discharge pre-treatment is high, and the use of dried shrimp shells in the prior art destroys a large amount of astaxanthin, and the production cycle is long. , clean production implementation is not thorough, etc., to provide a simple and effective method, that is, use the wet shrimp shells after boiling and removing the kernels as raw materials, combine enzymatic hydrolysis to extract protein, organic acid to extract organic calcium, and ethanol to extract astaxanthin , applied to the chitin production process to achieve clean production while extracting protein, astaxanthin, calcium and chitin from shrimp heads and shells

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