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Preparation method of Aralia elata bud polysaccharide

A technology of aralia aralia and polysaccharides, which is applied in the field of preparation of polysaccharide extracts, to achieve the effects of accelerating dissolution, simplifying the process, and high purity

Inactive Publication Date: 2016-04-27
HEILONGJIANG VOCATIONAL INST OF ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign research on Aralia aralia japonica mainly focuses on the extraction, separation, purification, and biological activity of saponins. It has been confirmed that it has exciting and strong effects on the human body, and can be used for acute and chronic inflammation and various nerves Weakness, but the research on the polysaccharides contained in it only found that the crude extraction content is relatively high, which is similar to the content in root bark and stem bark. In order to make better use of the resources of Aralia longya buds, we provide a high-efficiency, fast, simple and easy-to-operate preparation method, which is convenient for the refining and purification of Aralia longya bud polysaccharides in the future. Structural identification, biological activity research and application in actual production have become important issues to be solved at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Clean fresh wild Aralia japonica buds, cut them into 2-3cm sections, dry at 80°C for 24 hours to remove most of the water, then dry at 60°C for 48 hours, and crush them into coarse powder for later use;

[0025] (2) Take 5g of coarse powder, add 100ml of petroleum ether, degrease with ultrasonic power of 200W for 60min at room temperature, filter, and evaporate petroleum ether to obtain coarse powder after degreasing;

[0026] (3) Mix the degreased coarse powder with 150ml of purified water, add 50mg of cellulase, 75mg of pectinase, and 25mg of papain, stir evenly, shake in a water bath at 50°C for 120 minutes at a constant temperature, and filter to obtain a crude polysaccharide extract;

[0027] (4) Concentrate the crude polysaccharide extract to 15ml under reduced pressure. After cooling to room temperature, slowly add 60ml of 95% ethanol, stir while adding, let stand at 4°C for 12h, and centrifuge (6000r / min) to obtain a precipitate. After curing with water and ...

Embodiment 2

[0031] (1) Cleanly select fresh wild Aralia japonica buds, cut them into 2-3cm sections, dry at 80°C for 72 hours to remove most of the water, then dry at 60°C for 48 hours, and crush them into coarse powder for later use;

[0032] (2) Take 100g of coarse powder, add 1500ml of petroleum ether, degrease with ultrasonic power of 250W for 30min at room temperature, filter, and evaporate the petroleum ether to obtain coarse powder after degreasing;

[0033] (3) Mix the degreased coarse powder with 3500ml of purified water, add 2g of cellulase, 1.5g of pectinase, and 0.5g of papain, stir well, shake in a water bath at 55°C for 150min, and filter to obtain a crude polysaccharide extract;

[0034] (4) Concentrate the crude polysaccharide extract to 300ml under reduced pressure. After cooling to room temperature, slowly add 1200ml of 95% ethanol, stir while adding, let stand at 4°C for 12h, centrifuge (6000r / min) to obtain a precipitate, which is used for precipitation. Water and etha...

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Abstract

The invention relates to a preparation method of a polysaccharide extract, in particular to a preparation method of Aralia elata bud polysaccharide. Northeast wild Aralia elata buds are taken as raw materials, the raw materials are subjected to drying, crushing, degreasing with petroleum ether, water extraction with a compound enzyme, pigment and protein removal with macroporous resin, concentration, alcohol precipitation and centrifugation, precipitates are obtained, and a product is obtained through vacuum drying. The synergistic effect of the compound enzyme is applied to the extraction process of northeast wild Aralia elata bud polysaccharide for the first time, the condition is mild, the biological activity of the polysaccharide is guaranteed, the one-time extraction rate is high, ADS-7 strong polarity macroporous adsorption resin is used for removing impurities, pigments and protein can be removed efficiently, the operation is simple, and the obtained polysaccharide is high in purity, good in color and reconstitution property and suitable for industrial production and application.

Description

technical field [0001] The invention relates to a preparation method of a polysaccharide extract, in particular to a preparation method of aralia japonica bud polysaccharide. Background technique [0002] Araliaelata (Miq.) Seem., also known as Aralia elata (Miq.) Seem.), is a plant of the genus Aralia in Araliaceae. It is widely distributed in Heilongjiang, Jilin, Liaoning and other provinces and cities in my country, and is rich in wild resources. It can be used as medicine and food, the root bark and stem bark are used as medicine, and its tender shoots are eaten. Aralia longya buds are high-quality mountain wild vegetables for import and export, and the salted product can reach nearly 4,000 yuan per ton. It is a rare pollution-free and pure natural green food on the modern table. [0003] With the study of plant polysaccharides to enhance immune function, anti-radiation damage, anti-tumor and other bioactive functions, people have successfully applied polysaccharides to...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 张桂娟吴舜吕文博
Owner HEILONGJIANG VOCATIONAL INST OF ECOLOGICAL ENG
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