Amplification method, primer, sequencing method and mutation detection method of mitochondria whole genome DNA (Deoxyribonucleic Acid)

A whole-genome sequencing and whole-genome technology, applied in the field of molecular biology, can solve the problems of complicated operation and inability to detect copy number, etc., and achieve the effect of flexible cost and low cost
CN103173441AInactive Publication Date: 2013-06-26BGI GENOMICS CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BGI GENOMICS CO LTD
Publication Date
2013-06-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a primer pair and a kit for amplification of a mitochondria whole genome. The invention further discloses an amplification method, a sequencing method and a mutation detection method of the mitochondria whole genome DNA (Deoxyribonucleic Acid). According to the invention, the direct amplification of the given primer is combined with the direct sequencing method, so that a mitochondria whole genome sequence can be obtained; all mutations of the mitochondria genes can be detected by one step for being used for detecting the diseases caused by most of the mitochondria gene mutations.
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Description

technical field

[0001] The present invention relates to molecular biology, in particular to a primer pair set, a kit, an amplification method, a sequencing method and a mitochondrial DNA mutation detection method that can be used for the amplification of mitochondrial whole genome DNA. Background technique

[0002] The mitochondrial genome (mitochondrial DNA, mtDNA) is a closed double-stranded circular structure with a length of 16569 bp, which is divided into coding regions and non-coding regions. There are a total of 37 genes in the coding region, of which 22 encode transfer ribonucleic acid (tRNA), 2 encode ribosomal ribonucleic acid (12S and 16S rRNA), and 13 encode polypeptides. The mtDNA genes are closely arranged, and there are no introns between the sequences.

[0003] The molecular structure of mtDNA determines that it has the following unique genetic characteristics:

[0004] (1) High copy number. Generally speaking, there are only two copies of chromosomal DNA i...

Claims

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