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Protein identification method

An identification method and protein technology, which can be used in special data processing applications, instruments, electrical digital data processing, etc., and can solve problems such as incomplete protein data.

Active Publication Date: 2013-06-26
BGI TECH SOLUTIONS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are a large number of organisms that have not been sequenced, and their protein data are incomplete or even rare, which has caused great limitations to database-based protein identification methods

Method used

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  • Protein identification method
  • Protein identification method

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Experimental program
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Embodiment

[0036] The present invention is exemplarily described by taking mass spectral data of oysters dismounted from a Thermo Scientific LTQ Orbitrap Velos mass spectrometer as an example.

[0037] Currently, oysters do not have many protein data available, but there are 1287131 EST sequences (obtained from http: / / www.ncbi.nlm.nih.gov / nucest / ). Through Mascot (obtained from http: / / www.ncbi.nlm.nih.gov / nucest / ), these EST sequences were translated by six frames according to the codon rules to construct an EST amino acid sequence library (11488120 sequences). Then, Mascot searches were performed on the NCBInr animal protein database (2608768 sequences, obtained from http: / / www.ncbi.nlm.nih.gov / protein) and the constructed EST amino acid sequence library, respectively. The Mascot search parameters are as follows:

[0038] Mascot Search Parameters

[0039]

[0040]

[0041] The results of searching the above two databases are as follows:

[0042]

[0043] image 3 The histog...

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Abstract

The invention relates to a protein identification method based on an EST array, and a protein identification method based on a protein identification database and the EST array.

Description

technical field [0001] The present invention relates to the field of protein identification. More specifically, the present invention relates to database-based protein search identification methods. The invention also relates to a method for constructing a database for protein search and identification. Background technique [0002] With the rapid development of genomics (Genomics) and gene sequencing technology in the past ten years, the research on gene product proteins has gradually entered the field of vision of biologists. "It came into being. Proteome refers to all proteins corresponding to a genome, or all proteins expressed by cells and tissues under specific conditions and time. Proteomics refers to the discipline that studies the composition, structure, properties and functions of proteomes. The identification of proteins is the most basic and critical of the whole proteomics. [0003] At present, there are two main mass spectrometry-based technologies that ca...

Claims

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Application Information

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IPC IPC(8): G06F19/22
Inventor 周若闻博汪建王俊杨焕明
Owner BGI TECH SOLUTIONS
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